Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces toyocaensis |
Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzymes A319C and V425T in complex with substrate, hanging drop vapor diffusion method, using 140 mM ammonium acetate, 14% (w/v) PEG 4000, 100 mM sodium citrate pH 5.6 | Streptomyces toyocaensis |
Protein Variants | Comment | Organism |
---|---|---|
A319C | the catalytic efficiency of the mutant is about 1.5% of that of the wild type protein | Streptomyces toyocaensis |
V425T | the mutant shows reduced activity compared to the wild type enzyme | Streptomyces toyocaensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyphenylacetyl-amine-CoA | - |
Streptomyces toyocaensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | mutant enzyme A319C, at pH 7.5 and 24°C | Streptomyces toyocaensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis | - |
2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis A47934 | - |
2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces toyocaensis | Q8KLK7 | - |
- |
Streptomyces toyocaensis A47934 | Q8KLK7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Streptomyces toyocaensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | - |
Streptomyces toyocaensis | 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | - |
? | |
(3,5-dihydroxyphenyl)acetyl-CoA + O2 | - |
Streptomyces toyocaensis A47934 | 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Streptomyces toyocaensis |
Synonyms | Comment | Organism |
---|---|---|
DpgC | - |
Streptomyces toyocaensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | mutant enzyme A319C, at pH 7.5 and 24°C | Streptomyces toyocaensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is cofactor-independent | Streptomyces toyocaensis |