Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
structure at 1.73 A resolution. At the catalytic center, iron is ligated to His86, His90, Glu114, and two water molecules. Three conserved cysteines in the cavity are located 9.5-13 A from the iron and are observed as free thiol forms. The iron and Cys31 are essential | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
C101A | about 10% residual activtiy for both oxygenase and reductase activity | Sulfurisphaera tokodaii |
C104A | about 10% residual activtiy for both oxygenase and reductase activity | Sulfurisphaera tokodaii |
C31A | complete loss of activity | Sulfurisphaera tokodaii |
E114A | complete loss of activity | Sulfurisphaera tokodaii |
H86A | complete loss of activity | Sulfurisphaera tokodaii |
H90A | complete loss of activity | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sulfurisphaera tokodaii | |
cyanide | 1 mM, 71% inhibition | Sulfurisphaera tokodaii | |
dithiothreitol | inhibits both reactions | Sulfurisphaera tokodaii | |
Fe2+ | inhibits oxygenase reaction | Sulfurisphaera tokodaii | |
Fe3+ | inhibits oxygenase reaction | Sulfurisphaera tokodaii | |
Hg2+ | - |
Sulfurisphaera tokodaii | |
iodoacetic acid | inhibits both reactions | Sulfurisphaera tokodaii | |
additional information | not inhibitory: sodium azide | Sulfurisphaera tokodaii | |
N-ethylmaleimide | inhibits both reactions | Sulfurisphaera tokodaii | |
PCMB | inhibits both reactions | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | at the catalytic center, iron is ligated to His86, His90, Glu114, and two water molecules | Sulfurisphaera tokodaii | |
Zn2+ | activating | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
539000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, by heat treatment and chromatography steps with four different columns | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.95 | - |
80°C, pH 6, production of hydrogen sulfite | Sulfurisphaera tokodaii |
10.5 | - |
80°C, pH 6, oxygenase reaction | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3S + 3H2O | - |
Sulfurisphaera tokodaii | HSO3- + 2HS- + 3H+ | - |
? | |
4 sulfur + 4 H2O + O2 | - |
Sulfurisphaera tokodaii | 2 hydrogen sulfide + 2 sulfite | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | 15-16 * 38000, SDS-PAGE, 15-16 * 35700, calculated from sequence | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, the remaining oxygenase and reductase activities are approximately 40% and 90%, respectively | Sulfurisphaera tokodaii |