Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FLAP | required for activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of the mutant catalytically inactive DELTA13 isoform (5-LODELTA13) whose transcript lacks exon 13, intracellular localisation and phosphorylation profile of the human 5-lipoxygenase DELTA13 isoform differs from that of its full-length wild-type counterpart. Mutant 5-LODELTA13 inhibits 5-LO product biosynthesis when co-expressed with active wild-type 5-LO1. Mutant 5-LODELTA13 is hyperphosphorylated on S523 and S273 compared to wild-type 5-LO1. Mutant 5-LODELTA13 is cytosolic and concentrated in endoplasmic reticulum-rich perinuclear regions where its effect on LT biosynthesis may occur. Known regulatory factors are retained in the protein sequence of 5-LODELTA13., overview | Homo sapiens |
W102A | the mutant shows a diffuse cellular expression, while wild-type 5-LO1 is primarily located in the nucleoplasm | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleoplasm | wild-type enzyme 5-LO1 is primarily in the nucleoplasm, role for residue W102 in nuclear targeting of enzyme 5-LO1 | Homo sapiens | 5654 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09917 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | intracellular localisation and phosphorylation profile of the human 5-lipoxygenase DELTA13 isoform differs from that of full-length wild-type enzyme. Mutant 5-LODELTA13 is hyperphosphorylated on S523 and S273 compared to wild-type 5-LO1 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-lipoxygenase | - |
Homo sapiens |
5-LO | - |
Homo sapiens |
5-LO1 | - |
Homo sapiens |
ALOX5 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | catalytically inactive mutant 5-LODELTA13, lacking exon 13, inhibits 5-LO product biosynthesis when co-expressed with active wild-type 5-LO1. Mutant 5-LODELTA13 inhibits leukotriene but not 5-hydroxyeicosatetraenoic acid (5-HETE) biosynthesis. This inhibition is independent of 5-LODELTA13-FLAP interactions since it occurs in cells expressing FLAP or not. Mutant 5-LODELTA13 is hyperphosphorylated on S523 and S273 compared to wild-type 5-LO1 | Homo sapiens |