Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | Pseudomonas putida | - |
3-carboxy-cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protocol for the expression and purification of highly active Pseudomonas putida protocatechuate-3,4-dioxygenase with no detectable nuclease contamination. Purification by nickel affinity chromatography, elution in 2 steps of imidazole concentrations, chromatography fractions are analyzed by SDS-PAGE, fractions of nearly pure enzyme are concentrated and further purified by size exclusion chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | heterodimer of hexahistidine tagged pcaH and pcaG | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
PCD | - |
Pseudomonas putida |