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Literature summary for 1.13.11.27 extracted from

  • Bolognese, F.; Scanferla, C.; Caruso, E.; Orlandi, V.T.
    Bacterial melanin production by heterologous expression of 4'hydroxyphenylpyruvate dioxygenase from Pseudomonas aeruginosa (2019), Int. J. Biol. Macromol., 133, 1072-1080 .
    View publication on PubMed

Application

Application Comment Organism
synthesis overexpression of the HppD gene in Escherichia coli to produce large amounts of pyomelanin for biotechnological purposes. The recombinant dioxygenase expression leads to the production of pyomelanin in Escherichia coli. When cells are grown at the mid-exponential phase in a mineral medium with added glucose 10 mM and casamino acid 0.2 % w/v, the administration of tyrosine 1 mM after 30 minutes of exposure to arabinose 1 % w/v allows to purify 213 mg/l of pyomelanin after 6 days of biotransformation Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I576
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