Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 DE3 | Arabidopsis thaliana |
General Stability | Organism |
---|---|
the enzyme is stable for several hours at room temperature, indefinitely at 4°C | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(2-nitro-4-(trifluoromethyl)benzoyl)-1,3-cyclohexanedione | (NTBC), binding constant of 937 microM | Arabidopsis thaliana | |
mesotrione | binding constant of 602 microM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Kd value for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase Fe2+ is 29 microM, whereas the binding constant for phenylpyruvate is determined to be 319 microM | Arabidopsis thaliana | |
0.0069 | - |
4-hydroxyphenylpyruvate | activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C | Arabidopsis thaliana | |
0.115 | - |
oxygen | activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48650 | - |
- |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose anion exchange column, Sephacryl S-200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | 4-hydroxyphenylpyruvate dioxygenase does not use phenolpyruvate as a substrate in normal plant metabolism | Arabidopsis thaliana | homogentisate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPPD | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
4-hydroxyphenylpyruvate | activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C | Arabidopsis thaliana |