Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | 66% activity at 1 mM | Clostridium pasteurianum | |
CO | hydrogenase I is readily inhibited, hydrogenase II is irreversibly inactivated | Clostridium pasteurianum | |
CuSO4 | 0% activity at 1 mM | Clostridium pasteurianum | |
o-phenanthroline | 36% activity at 1 mM | Clostridium pasteurianum | |
O2 | 50% activity loss after 2 min | Clostridium pasteurianum | |
phenylmethanesulfonyl fluoride | 61% activity at 1 mM | Clostridium pasteurianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
reduced ferredoxin | hydrogen evolution, hydrogenase I | Clostridium pasteurianum | |
0.05 | - |
reduced ferredoxin | hydrogenase I, at pH 8.0 and 30°C | Clostridium pasteurianum | |
0.13 | - |
reduced ferredoxin | hydrogen evolution, hydrogenase II | Clostridium pasteurianum | |
0.13 | - |
reduced ferredoxin | hydrogenase II, at pH 8.0 and 30°C | Clostridium pasteurianum | |
0.18 | - |
oxidized methylene blue | hydrogen oxidation, hydrogenase I | Clostridium pasteurianum | |
0.18 | - |
oxidized methylene blue | hydrogenase I, at pH 8.0 and 30°C | Clostridium pasteurianum | |
0.31 | - |
oxidized methyl viologen | hydrogen evolution, hydrogenase II | Clostridium pasteurianum | |
0.31 | - |
oxidized methyl viologen | hydrogenase II, at pH 8.0 and 30°C | Clostridium pasteurianum | |
0.4 | - |
oxidized methylene blue | hydrogen oxidation, hydrogenase II | Clostridium pasteurianum | |
0.4 | - |
oxidized methylene blue | hydrogenase II, at pH 8.0 and 30°C | Clostridium pasteurianum | |
5 | - |
oxidized methyl viologen | hydrogen oxidation, hydrogenase I | Clostridium pasteurianum | |
5 | - |
oxidized methyl viologen | hydrogenase I, at pH 8.0 and 30°C | Clostridium pasteurianum | |
5.7 | - |
oxidized methyl viologen | hydrogen oxidation, hydrogenase II | Clostridium pasteurianum | |
5.7 | - |
oxidized methyl viologen | hydrogenase II, at pH 8.0 and 30°C | Clostridium pasteurianum | |
6.25 | - |
oxidized methyl viologen | hydrogen evolution, hydrogenase I | Clostridium pasteurianum | |
6.25 | - |
oxidized methyl viologen | hydrogenase I, at pH 8.0 and 30°C | Clostridium pasteurianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | hydrogenase I contains 12 atoms per mol enzyme and hydrogenase II 8 atoms per mol enzyme | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized ferredoxin | Clostridium pasteurianum | - |
H+ + reduced ferredoxin | - |
r | |
H2 + oxidized ferredoxin | Clostridium pasteurianum W5 | - |
H+ + reduced ferredoxin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Clostridium pasteurianum | - |
hydrogenase II, uptake hydrogenase | - |
Clostridium pasteurianum | - |
hydrogenase I, bidirectional hydrogenase | - |
Clostridium pasteurianum W5 | - |
- |
- |
Storage Stability | Organism |
---|---|
-180°C, 4 months, 100% activity | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + reduced ferredoxin | - |
Clostridium pasteurianum | H2 + oxidized ferredoxin | - |
r | |
H+ + reduced methyl viologen | - |
Clostridium pasteurianum | H2 + oxidized methyl viologen | - |
r | |
H2 + electron acceptor | methylene blue as electron acceptor | Clostridium pasteurianum | H+ + reduced electron acceptor | - |
? | |
H2 + electron acceptor | methyl viologen as electron acceptor | Clostridium pasteurianum | H+ + reduced electron acceptor | - |
? | |
H2 + electron acceptor | methylene blue as electron acceptor | Clostridium pasteurianum W5 | H+ + reduced electron acceptor | - |
? | |
H2 + electron acceptor | methyl viologen as electron acceptor | Clostridium pasteurianum W5 | H+ + reduced electron acceptor | - |
? | |
H2 + oxidized ferredoxin | - |
Clostridium pasteurianum | H+ + reduced ferredoxin | - |
r | |
H2 + oxidized ferredoxin | - |
Clostridium pasteurianum W5 | H+ + reduced ferredoxin | - |
r | |
H2 + oxidized methyl viologen | - |
Clostridium pasteurianum | H+ + reduced methyl viologen | - |
r | |
H2 + oxidized methyl viologen | - |
Clostridium pasteurianum W5 | H+ + reduced methyl viologen | - |
r | |
H2 + oxidized methylene blue | - |
Clostridium pasteurianum | H+ + reduced methylene blue | - |
r | |
H2 + oxidized methylene blue | - |
Clostridium pasteurianum W5 | H+ + reduced methylene blue | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
hydrogen evolution, hydrogenase II | Clostridium pasteurianum |
6.3 | - |
hydrogen evolution, hydrogenase I | Clostridium pasteurianum |
7 | - |
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor | Clostridium pasteurianum |
9.1 | - |
hydrogen evolution, hydrogenase II | Clostridium pasteurianum |
9.8 | - |
hydrogenase I, hydrogen oxidation | Clostridium pasteurianum |
10.5 | - |
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor | Clostridium pasteurianum |