Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1 mM, 97% inhibition | Pseudomonas putida | |
dithioerythritol | - |
Pseudomonas putida | |
FeCl3 | 0.1 mM, 45% inhibition | Pseudomonas putida | |
hydrazine | - |
Pseudomonas putida | |
hydroxylamine | - |
Pseudomonas putida | |
KCN | 0.1 mM, 82% inhibition | Pseudomonas putida | |
KF | - |
Pseudomonas putida | |
monoiodoamine | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
H2O2 | - |
Pseudomonas putida | |
4.4 | - |
Br- | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | contains 0.35 mol of cobalt ions per mol of enzyme. Addition of 0.1 mM CoCl2 results in 2.1fold increase in activity | Pseudomonas putida | |
Fe3+ | contains 2.0 mol of ferric iron per mol of enzyme | Pseudomonas putida | |
additional information | contains no vanadium ions | Pseudomonas putida | |
Ni2+ | contains 1.0 mol of nickel ions per mol of enzyme | Pseudomonas putida | |
Zn2+ | contains 0.8 mol of zinc ions per mol of enzyme | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000 | Pseudomonas putida |
68000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
IF-3 strain | - |
Pseudomonas putida IF-3 | - |
IF-3 strain | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.38 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Pseudomonas putida | ? | - |
? | |
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Pseudomonas putida IF-3 | ? | - |
? | |
I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Pseudomonas putida | ? | - |
? | |
I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Pseudomonas putida IF-3 | ? | - |
? | |
additional information | inactive toward C1- and F- in the monochlorodimedone assay system | Pseudomonas putida | ? | - |
? | |
additional information | inactive toward C1- and F- in the monochlorodimedone assay system | Pseudomonas putida IF-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000 | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
nonheme bromoperoxidase | - |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
20 | pH 7.0, 20 min, stable | Pseudomonas putida |
30 | 35 | pH 7.0, 20 min, 10% loss of activity | Pseudomonas putida |
40 | - |
pH 7.0, 20 min, 20% loss of activity | Pseudomonas putida |
50 | 60 | pH 7.0, 20 min, 10% loss of activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.4 | - |
2 h, 25°C, complete loss of activity below pH 3.5 | Pseudomonas putida |
4 | 11 | 2 h, 25°C, stable | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | contains no heme | Pseudomonas putida |