Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Paracoccus denitrificans |
Cloned (Comment) | Organism |
---|---|
gene ctaDII, which codes for subunit I of CcO, expression in Escherichia coli strain DH5alpha, the recombinant enzyme shows reduced catalase activity and thermal stability, overview. Expression of subunit I in the wild-type strain increases the catalase activity by 20fold. Overproduction of SU I might impair the correct insertion of heme a3 and CuB because of a deficiency in metal inserting chaperones. An altered distance between heme a3 and CuB and variations in protein structure are possible reasons for the observed increased catalase activity. Co-expression of chaperone-encoding genes genes ctaG and surf1c reduces the catalase activity of the organism, thermal stability is altered about aging, overview | Paracoccus denitrificans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the chromosomally encoded gene ctaDII (coding for subunit I present in aa3 CcO), is complemented on a low copy number plasmid controlled by the promoter of the cta operon | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Paracoccus denitrificans | - |
O2 + 2 H2O | - |
? | |
2 H2O2 | Paracoccus denitrificans AO1 | - |
O2 + 2 H2O | - |
? | |
additional information | Paracoccus denitrificans | bifunctional aa3 cytochrome c oxidase, CcO, possesses a peroxidase and a catalase activity | ? | - |
? | |
additional information | Paracoccus denitrificans AO1 | bifunctional aa3 cytochrome c oxidase, CcO, possesses a peroxidase and a catalase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | P08303 | cytochrome c oxidase with catalase activity; gene ctaG | - |
Paracoccus denitrificans AO1 | P08303 | cytochrome c oxidase with catalase activity; gene ctaG | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from membranes by solubilization with beta--dodecylmaltoside, immuno-affinity chromatography using a Strep-tagged antibody, and ultrafiltration. X-ray crystallography as well as mass spectrometry analysis reveal no signs for any covalent modification of subunit I besides the crosslink between H276 and Y280 | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Paracoccus denitrificans | O2 + 2 H2O | - |
? | |
2 H2O2 | - |
Paracoccus denitrificans AO1 | O2 + 2 H2O | - |
? | |
additional information | bifunctional aa3 cytochrome c oxidase, CcO, possesses a peroxidase and a catalase activity | Paracoccus denitrificans | ? | - |
? | |
additional information | bifunctional aa3 cytochrome c oxidase, CcO, possesses a peroxidase and a catalase activity | Paracoccus denitrificans AO1 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Paracoccus denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activit range | Paracoccus denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Paracoccus denitrificans |
General Information | Comment | Organism |
---|---|---|
malfunction | the recombinant enzyme shows reduced catalase activity and thermal stability, overview | Paracoccus denitrificans |
additional information | the enzyme's binuclear active center, residing in subunit I, contains heme a3 and CuB. Apart from its oxygen reductase activity, the protein possesses a peroxidase and a catalase activity | Paracoccus denitrificans |
physiological function | the catalase activity of CcO is clearly a side reaction | Paracoccus denitrificans |