Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | TvC-I and TvC-II, inactivation | Trigonopsis variabilis | |
CN- | TvC-I and TvC-II, at micromolar range | Trigonopsis variabilis | |
additional information | no reduction of TvC-II by dithionite | Trigonopsis variabilis | |
NaN3 | TvC-I and TvC-II, at micromolar range | Trigonopsis variabilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | TvC-II, heme-containing | Trigonopsis variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62900 | - |
4 * 62900, TvC-II, SDS-PAGE | Trigonopsis variabilis |
227300 | - |
TvC-II, gel filtration | Trigonopsis variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Trigonopsis variabilis | detoxification enzyme | O2 + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
chloroform | ethanol/chloroform: TvC-I and TvC-II, quite stable to | Trigonopsis variabilis |
Ethanol | ethanol/chloroform: TvC-I and TvC-II, quite stable to | Trigonopsis variabilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trigonopsis variabilis | - |
2 enzymes TvC-I and TvC-II | - |
Purification (Comment) | Organism |
---|---|
TvC-II, 300fold to homogeneity, TvC-I partially purified, TvC-I separation from TvC-II | Trigonopsis variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117 | - |
partially purified TvC-I | Trigonopsis variabilis |
55000 | - |
purified TvC-II | Trigonopsis variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Trigonopsis variabilis | O2 + H2O | - |
? | |
H2O2 | detoxification enzyme | Trigonopsis variabilis | O2 + H2O | - |
? | |
additional information | 2 enzymes TvC-I and TvC-II do not show peroxidase ctivity | Trigonopsis variabilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62900, TvC-II, SDS-PAGE | Trigonopsis variabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trigonopsis variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
TvC-I and TvC-II, rapid inactivation | Trigonopsis variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Trigonopsis variabilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | pH-profile, TvC-I | Trigonopsis variabilis |
4 | 10 | pH-profile, TvC-II | Trigonopsis variabilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | TvC-I, half-life: at least 50 h | Trigonopsis variabilis |
1 | 3 | TvC-I, half-life: about 2 h | Trigonopsis variabilis |
2 | - |
and below, TvC-I is rapidly inactivated | Trigonopsis variabilis |
3 | - |
TvC-I, half-life: at least 50 h | Trigonopsis variabilis |
4 | 10 | TvC-II is rapidly inactivated below pH 4.0 and above pH 10.0, at pH 6.0-10.0 the half-life is approximately 20 h, at pH 5.0 it is 45 h, at pH 4.0 77 h | Trigonopsis variabilis |