Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Hajipour, O.; Dogan, N.; Dincer, S.; Norizadehazehkand, M.
    Cloning, expression, and characterization of novel laccase enzyme from native Bacillus subtilis strain OH67 (2020), Mol. Biol. (Mosk.), 54, 611-617 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection the enzyme is able to decolorize efficiently a variety of chemical dyes, thus, being potentially applicable in textile and environmental industries Bacillus subtilis
industry the enzyme is able to decolorize efficiently a variety of chemical dyes, thus, being potentially applicable in textile and environmental industries Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 DE3 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1077
-
 guaiacol pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis OH67
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.3
-
 substrate: guaiacol, pH 7.5, 37°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Brilliant blue + O2
-
 Bacillus subtilis ?
-
 ?
Brilliant blue + O2
-
 Bacillus subtilis OH67 ?
-
 ?
guaiacol + O2
-
 Bacillus subtilis ?
-
 ?
guaiacol + O2
-
 Bacillus subtilis OH67 ?
-
 ?
Remazol brilliant orange 3R + O2
-
 Bacillus subtilis ?
-
 ?
Remazol brilliant orange 3R + O2
-
 Bacillus subtilis OH67 ?
-
 ?
Remazol red 106 + O2
-
 Bacillus subtilis ?
-
 ?
Remazol red 106 + O2
-
 Bacillus subtilis OH67 ?
-
 ?
Turquoise blue HF6 + O2
-
 Bacillus subtilis ?
-
 ?
Turquoise blue HF6 + O2
-
 Bacillus subtilis OH67 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: guaiacol Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 45% of maximal activity, 80°C: about 55% of maximal activity Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 1 h, 5% loss of activity Bacillus subtilis
20
-
 1 h, 4% loss of activity Bacillus subtilis
30
-
 1 h, 8% loss of activity Bacillus subtilis
40
-
 1 h, 1% loss of activity Bacillus subtilis
50
-
 1 h, no loss of activity Bacillus subtilis
60
-
 1 h, 9% loss of activity Bacillus subtilis
70
-
 1 h, 21% loss of activity Bacillus subtilis
80
-
 1 h, 44% loss of activity Bacillus subtilis
90
-
 1 h, 68% loss of activity Bacillus subtilis
100
-
 1 h, 80% loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 substrate: guaiacol Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 1 h, 79% loss of activity Bacillus subtilis
3.8
-
 1 h, 47% loss of activity Bacillus subtilis
4.6
-
 1 h, 23% loss of activity Bacillus subtilis
5.4
-
 1 h, 17% loss of activity Bacillus subtilis
6
-
 1 h, 1% loss of activity Bacillus subtilis
6.6
-
 1 h, no loss of activity Bacillus subtilis
7
-
 1 h, 2% loss of activity Bacillus subtilis
7.6
-
 1 h, 10% loss of activity Bacillus subtilis
8
-
 1 h, 48% loss of activity Bacillus subtilis
8.8
-
 1 h, 79% loss of activity Bacillus subtilis
9.4
-
 1 h, 86% loss of activity Bacillus subtilis
10
-
 1 h, 88% loss of activity Bacillus subtilis
10.4
-
 1 h, 91% loss of activity Bacillus subtilis