Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae using an improved signal peptide previously obtained and enzyme directed evolution | Agrocybe pediades |
Protein Variants | Comment | Organism |
---|---|---|
N398D | twice better activity at pH 6 and 1.2fold improvement at pH 3 | Agrocybe pediades |
V159E | two-fold improvement in the laccase activity detected at pH 3 and 6. Lower thermotolerance than native enzyme | Agrocybe pediades |
V159E/N398D | 1.4fold higher activity than mutant enzyme V159E and 2.6fold higher activity than mutant enzyme N398D with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as substrate. Lower thermotolerance than native enzyme | Agrocybe pediades |
V159E/N398D/I453F/M454L | parental-like activity at both pH values. Lower thermotolerance than native enzyme | Agrocybe pediades |
V159E/N398D/I453L/M454L | 1.3fold TAI at pH 3 and lower activity (0.8fold) than mutant enzyme V159E/N398D. Lower thermotolerance than native enzyme | Agrocybe pediades |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | mutant enzyme V159E/N398D | Agrocybe pediades | |
DMSO | mutant enzyme V159E/N398D | Agrocybe pediades | |
EDTA | mutant enzyme V159E/N398D | Agrocybe pediades | |
ethanol | mutant enzyme V159E/N398D | Agrocybe pediades | |
NaCl | mutant enzyme V159E/N398D | Agrocybe pediades | |
NaF | mutant enzyme V159E/N398D | Agrocybe pediades | |
SDS | mutant enzyme V159E/N398D | Agrocybe pediades |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
0.0046 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
0.016 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
0.08 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
0.53 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
1.7 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted by the fungus during lignocellulose degradation | Agrocybe pediades | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | multicopper oxidase | Agrocybe pediades |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrocybe pediades | - |
- |
- |
Agrocybe pediades AH40210 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | existence of three N-glycosylation sites in the laccase and their distinct effect on the secretion or catalytic activity of the enzyme is demonstrated | Agrocybe pediades |
Purification (Comment) | Organism |
---|---|
- |
Agrocybe pediades |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Agrocybe pediades | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Agrocybe pediades AH40210 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Agrocybe pediades | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Agrocybe pediades AH40210 | ? | - |
? | |
Evans Blue + O2 | activity with mutant enzyme V159E/N398D | Agrocybe pediades | ? | - |
? | |
guaiacol + O2 | - |
Agrocybe pediades | ? | - |
? | |
guaiacol + O2 | - |
Agrocybe pediades AH40210 | ? | - |
? | |
N,N-dimethyl-1,4-phenylenediamine + O2 | - |
Agrocybe pediades | ? | - |
? | |
N,N-dimethyl-1,4-phenylenediamine + O2 | - |
Agrocybe pediades AH40210 | ? | - |
? | |
p-phenylenediamine + O2 | - |
Agrocybe pediades | ? | - |
? | |
p-phenylenediamine + O2 | - |
Agrocybe pediades AH40210 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61.7 | - |
T50-value, mutant enzyme V159E/N398D/I453F/M454L | Agrocybe pediades |
62.5 | - |
T50-value, mutant enzyme V159E/N398D/I453L/M454L | Agrocybe pediades |
65.8 | - |
T50-value, mutant enzyme V159E | Agrocybe pediades |
65.8 | - |
T50-value, mutant enzyme V159E/N398D | Agrocybe pediades |
68.8 | - |
T50-value, mutant enzyme N398D | Agrocybe pediades |
69.9 | - |
T50-value, wild-type enzyme | Agrocybe pediades |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
0.08 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
1.7 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
6 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
33 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
56 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Agrocybe pediades |
4 | - |
oxidation of 2,6-dimethoxyphenol | Agrocybe pediades |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.051 | - |
pH and temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | NaF | |
0.7 | - |
pH and temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | SDS | |
74 | - |
pH and temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | NaCl | |
90 | - |
pH and temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | EDTA |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
107 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
371 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
388 | - |
N,N-dimethyl-1,4-phenylenediamine | pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
1490 | - |
2,6-dimethoxyphenol | pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades | |
7391 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E | Agrocybe pediades | |
75500 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D | Agrocybe pediades |