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Literature summary for 1.10.3.2 extracted from

  • Wikee, S.; Hatton, J.; Turbe-Doan, A.; Mathieu, Y.; Daou, M.; Lomascolo, A.; Kumar, A.; Lumyong, S.; Sciara, G.; Faulds, C.B.; Record, E.
    Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp (2019), Int. J. Mol. Sci., 20, 1864 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Aspergillus niger Pestalotiopsis sp. KF079

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.024
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.04
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.1
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.101
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.144
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.647
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 calculated from sequence Pestalotiopsis sp. KF079
63000
-
 calculated from sequence Pestalotiopsis sp. KF079

Organism

Organism UniProt Comment Textmining
Pestalotiopsis sp. KF079 A0A2U7VJL8
-
-
Pestalotiopsis sp. KF079 A0A2U7VJY0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pestalotiopsis sp. KF079 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Pestalotiopsis sp. KF079 ?
-
 ?
additional information guaiacol and tannic acid are poorly oxidized. Ferulic and or 3,4-dihydroxyphenylalanine (DOPA) are not oxidized at all. The industrially important dyes Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5 are more effciently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole Pestalotiopsis sp. KF079 ?
-
-
o-dianisidine + O2
-
 Pestalotiopsis sp. KF079 ?
-
 ?
syringaldazine + O2
-
 Pestalotiopsis sp. KF079 ?
-
 ?

Synonyms

Synonyms Comment Organism
PsLac1
-
 Pestalotiopsis sp. KF079
PsLac2
-
 Pestalotiopsis sp. KF079

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: 2,6-dimethoxyphenol Pestalotiopsis sp. KF079
55
-
 substrate: syringaldazine Pestalotiopsis sp. KF079
65
-
 substrate: syringaldazine Pestalotiopsis sp. KF079
70
-
 substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079
80
-
 substrate: 2,6-dimethoxyphenol Pestalotiopsis sp. KF079
80
-
 substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrate: 2,6-dimethoxyphenol Pestalotiopsis sp. KF079
20 80 20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079
35 60 35°C: about 30% of maximal activity, 60°C: about 35% of maximal activity, substrate: syringaldazine Pestalotiopsis sp. KF079
40 80 40°C: about 40% of maximal activity, 80°C: maximal activity, substrate: 2,6-dimethoxyphenol Pestalotiopsis sp. KF079
45 75 45°C: about 45% of maximal activity, 75°C: about 55% of maximal activity, substrate: syringaldazine Pestalotiopsis sp. KF079
50 80 50°C: about 50% of maximal activity, 80°C: maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme retains 100% activity after 3 h Pestalotiopsis sp. KF079

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.178
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.365
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.431
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.573
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.696
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
0.748
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
2.441
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
 substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079
3
-
 substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Pestalotiopsis sp. KF079
5
-
 substrate: 2,6-dimethoxyphenol Pestalotiopsis sp. KF079
5
-
 substrate: syringaldazine Pestalotiopsis sp. KF079

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 the enzyme is stable Pestalotiopsis sp. KF079

pI Value

Organism Comment pI Value Maximum pI Value
Pestalotiopsis sp. KF079 calculated from sequence
-
 4.13
Pestalotiopsis sp. KF079 calculated from sequence
-
 6.17

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.089
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
4.331
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
5.187
-
 o-Dianisidine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
5.672
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
9.102
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
24.532
-
 2,6-dimethoxyphenol 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
29.245
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH not specified in the publication Pestalotiopsis sp. KF079
82.936
-
 syringaldazine 30°C, pH not specified in the publication Pestalotiopsis sp. KF079