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Literature summary for 1.10.3.2 extracted from
- Kinetic characterization of purified laccase from Trametes hirsuta a study on laccase catalyzed biotransformation of 1,4-dioxane (2021), Biotechnol. Lett., 43, 613-626 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM, 1.42fold activation | Trametes hirsuta |  |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | advantageous for laccases in industrial application as it acts as broad pH range acting enzyme | Trametes hirsuta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | un competitive inhibition | Trametes hirsuta | |
| NaN3 | competitive inhibition | Trametes hirsuta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
| 0.4 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 5% inhibition | Trametes hirsuta | |
| Co2+ | 10 mM, 48% inhibition | Trametes hirsuta | |
| Cr2+ | 10 mM, 5% inhibition | Trametes hirsuta | |
| Fe2+ | 10 mM, 96% inhibition | Trametes hirsuta | |
| Hg2+ | 10 mM, 94% inhibition | Trametes hirsuta | |
| K+ | 10 mM, 82% inhibition | Trametes hirsuta | |
| Li+ | 10 mM, 47% inhibition | Trametes hirsuta | |
| Mn2+ | 10 mM, 32% inhibition | Trametes hirsuta | |
| Ni2+ | 10 mM, 31% inhibition | Trametes hirsuta | |
| Pb2+ | 10 mM, 72% inhibition | Trametes hirsuta | |
| Zn2+ | 10 mM, 7% inhibition | Trametes hirsuta | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes hirsuta | Q02497 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 54fold purification | Trametes hirsuta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 978.34 | - |
pH 5.0, temperature not specified in the publication | Trametes hirsuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-dioxane + O2 | the oxidation reaction is accelerated by 25, 22, 6 and 19% in presence of 1 mM syringaldehyde, vanillin, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and guaiacol mediators respectively | Trametes hirsuta | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta | ? | - |
? | |
| guaiacol + O2 | - |
Trametes hirsuta | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Trametes hirsuta |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 80% of maximal activity, 50°C: about 60% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Trametes hirsuta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | 16 h, less than 5% loss of activity | Trametes hirsuta |
| 40 | - |
16 h, about 65% loss of activity | Trametes hirsuta |
| 65 | - |
half-life: 5 h | Trametes hirsuta |
| 70 | - |
half-life: 4 h | Trametes hirsuta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 80.2 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
| 198.29 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Trametes hirsuta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | pH 3.0: about 75% of maximal activity, pH 8.0: about 60% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Trametes hirsuta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | stable | Trametes hirsuta |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
NaN3 | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
| 0.276 | - |
EDTA | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200.51 | - |
guaiacol | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
| 9914.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, temperature not specified in the publication | Trametes hirsuta | |
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