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Literature summary for 1.10.3.2 extracted from

  • Neelkant, K.S.; Shankar, K.; Jayalakshmi, S.K.; Sreeramulu, K.
    Purification, biochemical characterization, and facile immobilization of laccase from Sphingobacterium ksn-11 and its application in transformation of diclofenac (2020), Appl. Biochem. Biotechnol., 192, 831-844 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection the immobilized laccase transforms diclofenac to 4-OH diclofenac. The immobilized laccase can be used to transform or degrade several recalcitrant compounds from industrial effluents Sphingobacterium sp. ksn-11

Inhibitors

Inhibitors Comment Organism Structure
Al2+ 3 mM, 23% inhibition Sphingobacterium sp. ksn-11
Cu2+ 3 mM, 16% inhibition Sphingobacterium sp. ksn-11
EDTA 30% inhibition Sphingobacterium sp. ksn-11
Hg2+ 1 mM, 44% inhibition. 3 mM, 95% inhibition Sphingobacterium sp. ksn-11
Mg2+ 3 mM, 11% inhibition Sphingobacterium sp. ksn-11
Ni2+ 3 mM, 33% inhibition Sphingobacterium sp. ksn-11
Sodium azide 30% inhibition Sphingobacterium sp. ksn-11
Zn2+ 3 mM, 66% inhibition Sphingobacterium sp. ksn-11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.12
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 40°C Sphingobacterium sp. ksn-11
2.5
-
 guaiacol pH 4.5, 40°C Sphingobacterium sp. ksn-11
2.5
-
 syringaldazine pH 4.5, 40°C Sphingobacterium sp. ksn-11

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Sphingobacterium sp. ksn-11
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 1.6fold activation Sphingobacterium sp. ksn-11
Co2+ 1 mM, 1.28fold activation Sphingobacterium sp. ksn-11
Cu2+ 1 mM, 1.36fold activation Sphingobacterium sp. ksn-11
Fe2+ 1 mM, 1.57fold activation Sphingobacterium sp. ksn-11
Mg2+ 1 mM, 1.44fold activation Sphingobacterium sp. ksn-11
Ni2+ 1 mM, 1.14fold activation Sphingobacterium sp. ksn-11

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
-
 Sphingobacterium sp. ksn-11

Organism

Organism UniProt Comment Textmining
Sphingobacterium sp. ksn-11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobacterium sp. ksn-11

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
377.2
-
 pH 4.5, 40°C Sphingobacterium sp. ksn-11

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Sphingobacterium sp. ksn-11 ?
-
 ?
guaiacol + O2
-
 Sphingobacterium sp. ksn-11 ?
-
 ?
syringaldazine + O2
-
 Sphingobacterium sp. ksn-11 ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Sphingobacterium sp. ksn-11

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Sphingobacterium sp. ksn-11

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 incubation of enzyme above 55 °C initiates denaturing of enzyme and conformational changes, leveraging the decreased enzyme activity Sphingobacterium sp. ksn-11

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Sphingobacterium sp. ksn-11
7
-
 substrate: syringaldazine Sphingobacterium sp. ksn-11