Cloned (Comment) | Organism |
---|---|
- |
Polyporus brumalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-coumaric acid | 32.5% residual activity at 1 mM | Polyporus brumalis | |
Ca2+ | 89.8% residual activity at 1 mM | Polyporus brumalis | |
Cd2+ | 88.0% residual activity at 1 mM | Polyporus brumalis | |
Co2+ | 75.3% residual activity at 01 mM | Polyporus brumalis | |
dithiothreitol | completely inhibited by 1.0 mM dithiothreitol | Polyporus brumalis | |
EDTA | 50% residual activity at 10 mM | Polyporus brumalis | |
K+ | 65.1% residual activity at 10 mM | Polyporus brumalis | |
kojic acid | 27.7% residual activity at 1 mM | Polyporus brumalis | |
L-cysteine | completely inhibited by 1.0 mM L-cysteine | Polyporus brumalis | |
Mg2+ | 68.8% residual activity at 10 mM | Polyporus brumalis | |
Mn2+ | 82.3% residual activity at 10 mM | Polyporus brumalis | |
Na+ | 56.6% residual activity at 10 mM | Polyporus brumalis | |
NaN3 | 8.9% residual activity at 1 mM | Polyporus brumalis | |
Sn2+ | 4.6% residual activity at 1 mM | Polyporus brumalis | |
tropolone | 9.7% residual activity at 1 mM | Polyporus brumalis | |
Zn2+ | 65.7% residual activity at 1 mM | Polyporus brumalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis | |
0.07 | - |
2,6-dimethoxyphenol | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis | |
0.12 | - |
ferulic acid | in McIlvaine buffer (pH 5.0), at 30°C | Polyporus brumalis | |
0.27 | - |
4-phenylenediamine | in McIlvaine buffer (pH 5.0), at 30°C | Polyporus brumalis | |
0.56 | - |
pyrogallol | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis | |
0.66 | - |
3,4-dihydroxy L-phenylalanine | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis | |
8.06 | - |
catechol | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis | |
10.7 | - |
guaiacol | in McIlvaine buffer (pH 4.0), at 30°C | Polyporus brumalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 138% activity at 10 mM | Polyporus brumalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63400 | - |
estimated from SDS-PAGE | Polyporus brumalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Polyporus brumalis | A3F8Z8 | - |
- |
Polyporus brumalis ibrc05015 | A3F8Z8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sepharose Q column chromatography | Polyporus brumalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Polyporus brumalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Polyporus brumalis | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Polyporus brumalis | ? | - |
? | |
3,4-dihydroxy L-phenylalanine + O2 | - |
Polyporus brumalis | ? | - |
? | |
4-phenylenediamine + O2 | - |
Polyporus brumalis | ? | - |
? | |
catechol + O2 | - |
Polyporus brumalis | ? | - |
? | |
catechol + O2 | - |
Polyporus brumalis ibrc05015 | ? | - |
? | |
ferulic acid + O2 | - |
Polyporus brumalis | ? | - |
? | |
ferulic acid + O2 | - |
Polyporus brumalis ibrc05015 | ? | - |
? | |
guaiacol + O2 | - |
Polyporus brumalis | ? | - |
? | |
additional information | Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator | Polyporus brumalis | ? | - |
? | |
additional information | Lac1 does not oxidize L-tyrosine | Polyporus brumalis | ? | - |
? | |
additional information | Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator | Polyporus brumalis ibrc05015 | ? | - |
? | |
additional information | Lac1 does not oxidize L-tyrosine | Polyporus brumalis ibrc05015 | ? | - |
? | |
pyrogallol + O2 | - |
Polyporus brumalis | ? | - |
? | |
pyrogallol + O2 | - |
Polyporus brumalis ibrc05015 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAC1 | - |
Polyporus brumalis |
p-benzenediol:dioxygen oxidoreductase | - |
Polyporus brumalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at pH. 4.0 | Polyporus brumalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | - |
Polyporus brumalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | the Lac activity of Lac1 remains 80% after incubation at 50°C for 30 min. However, incubated at 60°C, the remaining activity declines in less than 80%. The enzyme is inactive at 70-80°C | Polyporus brumalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Polyporus brumalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | - |
Polyporus brumalis |