Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 131.1% relative activity in the presence of 2 mM SDS | Fusarium solani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 20 mM | Fusarium solani | |
Cd2+ | 71.1% residual activity at 20 mM | Fusarium solani | |
Co2+ | 61.9% residual activity at 20 mM | Fusarium solani | |
Cr3+ | 46.2% residual activity at 20 mM | Fusarium solani | |
Cu2+ | 68.9% residual activity at 20 mM | Fusarium solani | |
dithiothreitol | complete inhibition at 2 mM | Fusarium solani | |
EDTA | 20.8% residual activity at 20 mM | Fusarium solani | |
Fe2+ | 4.0% residual activity at 20 mM | Fusarium solani | |
Fe3+ | complete inhibition at 20 mM | Fusarium solani | |
Mn2+ | 49.1% residual activity at 20 mM | Fusarium solani | |
NaN3 | complete inhibition at 2 mM | Fusarium solani | |
Ni2+ | 62% residual activity at 20 mM | Fusarium solani | |
Pb2+ | 97.2% residual activity at 20 mM | Fusarium solani | |
SDS | 93.8% residual activity at 20 mM | Fusarium solani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
0.0794 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
2.657 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
3.434 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Fusarium solani | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 130.7% relative activity in the presence of 20 mM Hg2+ | Fusarium solani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
SDSPAGE | Fusarium solani |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | the laccase retains about 60% of its activity in the presence of 5% (v/v) acetone | Fusarium solani |
acetonitrile | the laccase retains about 75% of its activity in the presence of 5% (v/v) acetonitrile | Fusarium solani |
DMSO | the laccase retains about 70% of its activity in the presence of 5% (v/v) DMSO | Fusarium solani |
Ethanol | the laccase retains about 75% of its activity in the presence of 5% (v/v) ethanol | Fusarium solani |
Methanol | the laccase retains more than 75-80% of its activity in the presence of 5% (v/v) methanol | Fusarium solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani | - |
- |
- |
Fusarium solani MAS2 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Fusarium solani |
Purification (Comment) | Organism |
---|---|
HiTrap DEAE column chromatography and Sephacryl S-200 gel filtration | Fusarium solani |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.19 | - |
crude culture, at 30°C, pH 4.5 | Fusarium solani |
358.7 | - |
after 69.1fold purification, at 30°C, pH 4.5 | Fusarium solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Fusarium solani | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | best substrate | Fusarium solani | ? | - |
? | |
2,6-dimethoxyphenol + O2 | best substrate | Fusarium solani MAS2 | ? | - |
? | |
catechol + O2 | - |
Fusarium solani | ? | - |
? | |
catechol + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
guaiacol + O2 | - |
Fusarium solani | ? | - |
? | |
guaiacol + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
syringaldazine + O2 | - |
Fusarium solani | ? | - |
? | |
syringaldazine + O2 | - |
Fusarium solani MAS2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, native PAGE | Fusarium solani |
Synonyms | Comment | Organism |
---|---|---|
benzenediol: oxygen oxidoreductase | - |
Fusarium solani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Fusarium solani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme retains activity after incubation at 50°C for 1 h, but the activity sharply decreases when the temperature is increased to 60°C and almost no activity is detected at 70°C. The laccase can still oxidize 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) when incubated at 70°C for 5 min, but when the enzyme is incubated under the same condition without substrate, it loses almost all its activity | Fusarium solani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.4 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
44.9 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
107.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
121 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Fusarium solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | the purified laccase maintains stable at pH 3.0-11.0. The enzyme remains active at pH values from 3.0 to 12.0, and its activity at pH 2.0 and 12.0 are 56.6% and 85.5% of that at pH 7.0, respectively | Fusarium solani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
13 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
1300 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
14000 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani |