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Literature summary for 1.1.1.B20 extracted from

  • Russmayer, H.; Marx, H.; Sauer, M.
    Microbial 2-butanol production with Lactobacillus diolivorans (2019), Biotechnol. Biofuels, 12, 262 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.B20

Protein Variants

Protein Variants Comment Organism
additional information the enzyme from Serratia marcescens is overexpressed in Lactobacillus diolivorans to produce meso-2,3-butanediol (meso-2,3-BTD). A two-step cultivation process with Serratia marcescens is developed for production of 2-butanol in Lactobacillus diolivorans via vitamin B12-dependent diol dehydratase (PduCDE) and alcohol dehydrogenase (pduQ). In the first step of the process, Serratia marcescens is used to produce stereospecifically meso-2,3-BTD from glucose followed by heat inactivation of Serratia marcescens. The accumulated meso-2,3-BTD is then converted during anaerobic fermentation with glucose into 2-butanol by Lactobacillus diolivorans. The process yields a butanol concentration of 10 g/l relying on wild-type bacterial strains. A further improvement of the maximum butanol titer is achieved using an engineered Lactobacillus diolivorans strain overexpressing the endogenous alcohol dehydrogenase pduQ. The two-step cultivation process based on this engineered strain leads to a maximum 2-butanol titer of 13.4 g/l, which means an increase of 34% Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+ Serratia marcescens
-
 (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+ Serratia marcescens DSMZ 14187
-
 (3R)-acetoin + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Serratia marcescens H9XP47
-
-
Serratia marcescens DSMZ 14187 H9XP47
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information batch cultivations with glucose as sole carbon source are conducted. Acetate is co-utilised by Serratia marcescens as carbon source Serratia marcescens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+
-
 Serratia marcescens (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+
-
 Serratia marcescens DSMZ 14187 (3R)-acetoin + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase UniProt Serratia marcescens
budC
-
 Serratia marcescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Serratia marcescens

General Information

General Information Comment Organism
additional information Serratia marcescens is a very efficient producer of meso-2,3-butanediol (meso-2,3-BTD)from glucose Serratia marcescens