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Literature summary for 1.1.1.427 extracted from

  • Brouns, S.J.; Walther, J.; Snijders, A.P.; van de Werken, H.J.; Willemen, H.L.; Worm, P.; de Vos, M.G.; Andersson, A.; Lundgren, M.; Mazon, H.F.; van den Heuvel, R.H.; Nilsson, P.; Salmon, L.; de Vos, W.M.; Wright, P.C.; Bernander, R.; van der Oost, J.
    Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment (2006), J. Biol. Chem., 281, 27378-27388.
    View publication on PubMed
Search for more literature for 1.1.1.427

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADP+ pH 7.5, 80°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two zinc ions per monomer Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37291
-
 4 * 37291, calculated from sequence Saccharolobus solfataricus
149700
-
 mass spectrometry Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabinose + NADP+ Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+ Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway D-arabinono-1,4-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YM2
-
-
Saccharolobus solfataricus P2 Q97YM2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NADP+
-
 Saccharolobus solfataricus D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+ the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+
-
 Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+ the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-ribose + NADP+
-
 Saccharolobus solfataricus D-ribonolactone + NADPH
-
 ?
D-ribose + NADP+
-
 Saccharolobus solfataricus P2 D-ribonolactone + NADPH
-
 ?
L-fucose + NADP+ most active with L-fucose as substrate Saccharolobus solfataricus L-fuconolactone + NADPH + H+
-
 ?
L-fucose + NADP+ most active with L-fucose as substrate Saccharolobus solfataricus P2 L-fuconolactone + NADPH + H+
-
 ?
L-galactose + NADP+
-
 Saccharolobus solfataricus L-galactonolactone + NADPH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37291, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Sso1300
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
74 100 more than 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 half-life: 42 min Saccharolobus solfataricus
90
-
 half-life: 26 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.7
-
 D-ribose pH 7.5, 80°C Saccharolobus solfataricus
17.7
-
 L-galactose pH 7.5, 80°C Saccharolobus solfataricus
23.8
-
 D-arabinose pH 7.5, 80°C Saccharolobus solfataricus
26.8
-
 L-fucose pH 7.5, 80°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus
8.2
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.3 more than 50% of maximal activity Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NADP+ the Km-value for NAD+ is 30fold higher compared to the Km-value for NADP+ Saccharolobus solfataricus