Cloned (Comment) | Organism |
---|---|
gene atlD, cloning of genes coding for arabitol utilization pathway, arabitol utilization genes are co-transcribed in an operon, a gene cluster comprising eight genes that is upregulated during growth with arabitol as a sole carbon source, transcriptional organization of the gene cluster, transcriptome sequencing, and real-time quantitative reverse transcription PCR enzyme expression analysis. Recombinant expression of atlD in Corynebacterium glutamicum strain ATCC 13032 mtlD deletion mutant complements the mutant and restores growth of the Corynebacterium glutamicum DELTAmtlD mutant with arabitol | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol 1-phosphate + NAD+ | Bacillus methanolicus | - |
D-xylulose 5-phosphate + NADH + H+ | - |
? | |
D-arabitol 1-phosphate + NAD+ | Bacillus methanolicus MGA3 | - |
D-xylulose 5-phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
- |
- |
Bacillus methanolicus MGA3 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:D-arabitol-grown cell | D-arabitol is a carbon source of Bacillus methanolicus | Bacillus methanolicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
crude extracts of Bacillus methanolicus cells grown on arabitol, pH 7.2, 30°C | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol 1-phosphate + NAD+ | - |
Bacillus methanolicus | D-xylulose 5-phosphate + NADH + H+ | - |
? | |
D-arabitol 1-phosphate + NAD+ | - |
Bacillus methanolicus MGA3 | D-xylulose 5-phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arabitol phosphate dehydrogenase | - |
Bacillus methanolicus |
atlD | - |
Bacillus methanolicus |
D-arabitol phosphate dehydrogenase | - |
Bacillus methanolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus methanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bacillus methanolicus |
Organism | Comment | Expression |
---|---|---|
Bacillus methanolicus | the enzyme is induced by D-arabitol | up |
General Information | Comment | Organism |
---|---|---|
metabolism | identification of genes coding for arabitol utilization pathway, overview. Bacillus methanolicus possesses an arabitol inducible operon encoding, amongst others, a putative PTS system and an arabitol phosphate dehydrogenase for uptake and activation of arabitol as growth substrate. Model of the pathways for arabitol catabolism in bacteria, overview | Bacillus methanolicus |