Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q04828 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ | residues Leu/Val54, Tyr55, His117, Val128, Ile129, His222, and Trp227 are involved in substrate binding, Tyr55, Asp50, Lys84, and His117 form the catalytic tetrad, Tyr55 and His117 together with NADP+ form an oxyanion hole through hydrogen bond networks, molecular docking simulations, detailed overview | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0039 | - |
with substrate 5alpha-dihydrotestosterone, formation of the 3alpha-hydroxysteroid | Homo sapiens |
0.0161 | - |
with substrate 5alpha-dihydrotestosterone, formation of the 3beta-hydroxysteroid | Homo sapiens |
0.0312 | - |
with substrate tibolone | Homo sapiens |
0.0432 | - |
with substrate progesterone | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH + H+ | i.e. DHT, reductive 3alpha-HSD activity of AKR1C1 yielding the 3alpha,17-diol, low activity | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
5alpha-dihydrotestosterone + NADPH + H+ | i.e. DHT, reductive 3beta-HSD activity of AKR1C1 yielding the 3beta,17-diol, preferred reaction | Homo sapiens | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
? | |
additional information | AKR1C1 is a cytosolic hydroxysteroid dehydrogenase, that reduces 3-ketosteroids and 20-ketosteroids, overview, molecular docking simulations with generation of docking targets, overview | Homo sapiens | ? | - |
? | |
progesterone + NADPH | i.e. pregn-4-ene-3,20-dione | Homo sapiens | pregn-4-ene-20alpha-ol-3-one + NADP+ | - |
? | |
[7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-one + NADPH | i.e. tibolone, a synthetic hormone | Homo sapiens | [7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-ol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid reductase | - |
Homo sapiens |
3beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |
AKR1C1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |