Cloned (Comment) | Organism |
---|---|
gene ldhB, from strain NRRL 395 and 99-880, expression of His6-tagged LdhB in Escherichia coli strain BL21(DE3) | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | Rhizopus arrhizus | LdhB enzymes from type-I isolate NRRL 395 and type-II isolate 99-880 show reductive LDH activity, but no oxidative LDH activity, overview | (S)-lactate + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | - |
strains 99-880 and NRRL 395, genes ldhB and ldhA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged LdhB from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography and cleavage of the tag | Rhizopus arrhizus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | type-I isolate, e.g. strain NRRL 395, accumulating primarily lactic acid when grown in the presence of a fermentable carbon source and containing two LDH genes, ldhA and ldhB, and type-II isolates, e.g. strain 99-880, synthesizing predominantly fumaric acid and having only an ldhB gene | Rhizopus arrhizus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | LdhB enzymes from type-I isolate NRRL 395 and type-II isolate 99-880 show reductive LDH activity, but no oxidative LDH activity, overview | Rhizopus arrhizus | (S)-lactate + NAD+ | - |
ir | |
pyruvate + NADH + H+ | LdhB | Rhizopus arrhizus | (S)-lactate + NAD+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
LdhA | - |
Rhizopus arrhizus |
LDHB | - |
Rhizopus arrhizus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhizopus arrhizus | |
NADH | - |
Rhizopus arrhizus |