Cloned (Comment) | Organism |
---|---|
protein expression of Y63 using GAL promoter of pYes2NT vector in yeast | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
net Y63 protein | Saccharomyces cerevisiae |
45000 | - |
partially purified protein of the histidineY63 complex | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
This UniProt-ID has been deleted; NRRL Y-12632 | - |
Saccharomyces cerevisiae NRRL Y-12632 | - |
This UniProt-ID has been deleted; NRRL Y-12632 | - |
Purification (Comment) | Organism |
---|---|
overexpressed Y63 protein partially purified by Ni-NTA column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
with 4-methoxybenzaldehyde as substrate | Saccharomyces cerevisiae |
0.58 | - |
with 5-(hydroxymethyl)furan-2-carbaldehyde as substrate | Saccharomyces cerevisiae |
0.9 | - |
with acetaldehyde as substrate | Saccharomyces cerevisiae |
0.99 | - |
with trans-2-nonenaldehyde as substrate | Saccharomyces cerevisiae |
1.28 | - |
with cinnamic aldehyde as substrate | Saccharomyces cerevisiae |
1.31 | - |
with benzoic aldehyde as substrate | Saccharomyces cerevisiae |
3.63 | - |
with octanaldehyde as substrate | Saccharomyces cerevisiae |
3.73 | - |
with heptanaldehyde as substrate | Saccharomyces cerevisiae |
3.97 | - |
with phenylacetaldehyde as substrate | Saccharomyces cerevisiae |
4.19 | - |
with with furan-2-carbaldehyde as substrate | Saccharomyces cerevisiae |
4.99 | - |
with valeraldehyde as substrate | Saccharomyces cerevisiae |
6.34 | - |
with butyraldehyde as substrate | Saccharomyces cerevisiae |
6.41 | - |
with propionaldehyde as substrate | Saccharomyces cerevisiae |
7.67 | - |
with hexanaldehyde as substrate | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 4-methoxybenzyl alcohol + NADP+ | - |
? | |
4-methoxybenzaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | 4-methoxybenzyl alcohol + NADP+ | - |
? | |
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | (furan-2,5-diyl)dimethanol + NADP+ | - |
? | |
5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | (furan-2,5-diyl)dimethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ethanol + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | ethanol + NADP+ | - |
? | |
benzoic aldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
butyraldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
cinnamic aldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
furan-2-carbaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
heptanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
hexanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
additional information | the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus | Saccharomyces cerevisiae NRRL Y-12632 | ? | - |
? | |
octanaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
phenylacetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | 2-phenylethanol + NADP+ | - |
? | |
phenylacetaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae NRRL Y-12632 | 2-phenylethanol + NADP+ | - |
? | |
propionaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | propanol + NADP+ | - |
? | |
trans-2-nonenaldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
valeraldehyde + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent aldehyde reductase | - |
Saccharomyces cerevisiae |
Y63 protein | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | prefered | Saccharomyces cerevisiae |