Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.192 extracted from

  • Seo, E.J.; Kim, H.J.; Kim, M.J.; Kim, J.S.; Park, J.B.
    Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids (2019), Chem. Commun. (Camb.), 55, 14462-14465 .
    View publication on PubMed
Search for more literature for 1.1.1.192

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Micrococcus luteus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.0 M trisodium citrate dihydrate at pH 6.5 Micrococcus luteus

Protein Variants

Protein Variants Comment Organism
D37S/A38R/V39S the mutant has preferred activity with NADP+ and shows increased catalytic efficiency compared to the wild type enzyme Micrococcus luteus
D37S/V39S the mutant has also activity with NADP+ and shows increased catalytic efficiency compared to the wild type enzyme Micrococcus luteus
T15I/D37S/A38R/V39S the mutant shows about 10fold increased catalytic efficiency with 10-hydroxyoctadecanoic acid and has 1800fold increase in catalytic efficiency for NADP+ compared to the wild type enzyme Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10,12-dihydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus ?
-
 ?
10,12-dihydroxyoctadecanoic acid + NADP+ + H2O the reaction is catalyzed by mutant enzyme D37S/V39S Micrococcus luteus ? + NADPH
-
 ?
10-hydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus 9-(nonanoyloxy)nonanoic acid + NADH
-
 ?
10-hydroxyoctadecanoic acid + NADP+ + H2O the reaction is catalyzed by mutant enzyme D37S/A38R/V39S/T15I Micrococcus luteus 9-(nonanoyloxy)nonanoic acid + NADPH
-
 ?
12-hydroxyoctadecanoic acid + NAD+ + H2O
-
 Micrococcus luteus ? + NADH
-
 ?
4-decanol + NAD+ + H2O
-
 Micrococcus luteus ? + NADH
-
 ?
additional information the wild type enzyme shows no activity with NADP+ Micrococcus luteus ?
-
-

Synonyms

Synonyms Comment Organism
NAD+-dependent secondary alcohol dehydrogenase
-
 Micrococcus luteus
SADH
-
 Micrococcus luteus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Micrococcus luteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00061
-
 12-hydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.0014
-
 10-hydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.0014
-
 10-hydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.0015
-
 12-hydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.0021
-
 10,12-dihydroxyoctadecanoic acid mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.0035
-
 10,12-dihydroxyoctadecanoic acid wild type enzyme, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.012
-
 10,12-dihydroxyoctadecanoic acid mutant enzyme D37S/V39S, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus
0.013
-
 10-hydroxyoctadecanoic acid mutant enzyme D37S/A38R/V39S/T15I, with NAD+ as cosubstrate, pH and temperature not specified in the publication Micrococcus luteus