Cloned (Comment) | Organism |
---|---|
gene nosE, overexpression in Escherichia coli BL21(DE3) as N-terminally His-tagged enzyme | Nostoc sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
(2S,4S)-5-hydroxyleucine | pH 10.0, 42°C, recombinant enzyme | Nostoc sp. | |
1.2 | - |
(2S)-5-hydroxy-2-aminovaleric acid | pH 10.0, 42°C, recombinant enzyme | Nostoc sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Nostoc sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, about, recombinant enzyme, SDS-PAGE | Nostoc sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-5-hydroxyleucine + NAD+ + H2O | Nostoc sp. | enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline | (4S)-5-oxo-L-leucine + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | - |
GSV224, gene nosE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography | Nostoc sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-5-hydroxy-2-aminovaleric acid + NAD+ + H2O | no activity with the (2R)-isomer | Nostoc sp. | (2S)-2-amino-5-oxopentanoate + NADH | - |
? | |
(2S,4S)-5-hydroxyleucine + NAD+ + H2O | - |
Nostoc sp. | (4S)-5-oxo-L-leucine + NADH | - |
r | |
(2S,4S)-5-hydroxyleucine + NAD+ + H2O | enzyme reaction is part of the biosynthesis of (2S,4S)-4-methylproline | Nostoc sp. | (4S)-5-oxo-L-leucine + NADH | - |
r | |
additional information | stereospecific abstraction of the 5pro-S hydrogen | Nostoc sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, about, recombinant enzyme, SDS-PAGE | Nostoc sp. |
Synonyms | Comment | Organism |
---|---|---|
long-chain dehydrogenase | - |
Nostoc sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Nostoc sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Nostoc sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for the A-side of NAD+, no activity with NADP+ | Nostoc sp. |