Cloned (Comment) | Organism |
---|---|
expression of 3beta HSD I and III in COS-1 cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
pregnenolone | 3betaHSD I | Mus musculus | |
0.00014 | - |
dehydroepiandrosterone | 3betaHSD I | Mus musculus | |
0.00016 | - |
androstanediol | 3betaHSD I | Mus musculus | |
0.00046 | - |
androstanediol | 3betaHSD III | Mus musculus | |
0.00049 | - |
dehydroepiandrosterone | 3betaHSD III | Mus musculus | |
0.00103 | - |
pregnenolone | 3betaHSD III | Mus musculus | |
0.0055 | - |
stanolone | 3betaHSD I | Mus musculus | |
0.0068 | - |
stanolone | 3betaHSD III | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroepiandrosterone + NAD+ | Mus musculus | - |
androst-5-en-3,17-dione + NADH + H+ | - |
? | |
pregnenolone + NAD(P)+ | Mus musculus | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | 3betaHSD I | Mus musculus | - |
kidney | 3betaHSD II and III | Mus musculus | - |
liver | 3betaHSD II and III | Mus musculus | - |
ovary | 3betaHSD I | Mus musculus | - |
testis | 3betaHSD I and VI | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Mus musculus | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
5alpha-androstanediol + NAD+ | - |
Mus musculus | 5alpha-dihydrotestosterone + NADH | - |
r | |
androstanediol + NAD+ | - |
Mus musculus | dihydrotestosterone + NADH + H+ | - |
r | |
dehydroepiandrosterone + NAD+ | - |
Mus musculus | androst-5-en-3,17-dione + NADH + H+ | - |
? | |
pregnenolone + NAD(P)+ | - |
Mus musculus | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |