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GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-galactose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
9% of the specific activity with 1,2-diacyl-sn-glycerol
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-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol + UDP
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?
UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
additional information
?
-
GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
GDP-glucose shows poor activity
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-
?
GDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
GDP-glucose shows poor activity
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor. Can form tetraglucosyl diacylglycerol in vitro
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-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor. Can form tetraglucosyl diacylglycerol in vitro
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
2.2% of the specific activity with 1,2-diacyl-sn-glycerol
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
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-
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor. Can form tetraglucosyl diacylglycerol in vitro
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor. Can form tetraglucosyl diacylglycerol in vitro
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
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main product
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?
UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
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?
UDP-alpha-D-glucose + glycero-3-phospho-(1'-sn-glycerol)
alpha-D-glucopyranosyl-glycero-3-phospho-(1'-sn-glycerol) + UDP
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?
additional information
?
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enzyme transfers glucosyl and galactosyl residues from UDP-Glc and UDP-Gal to dioleoylglycerol acceptor to form the corresponding beta-glycosyl-dioleoylglycerol, which then acts as acceptor to give beta-diglycosyl-dioleoylglycerol products. Enzyme sequentially adds glycosyl residues with beta-1,6 connectivity, the first glycosyltransferase activity to dioleoylglycerol being about 1 order of magnitude higher than the second to monoglucosyldioleoylglycerol
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?
additional information
?
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enzyme transfers glucosyl and galactosyl residues from UDP-Glc and UDP-Gal to dioleoylglycerol acceptor to form the corresponding beta-glycosyl-dioleoylglycerol, which then acts as acceptor to give beta-diglycosyl-dioleoylglycerol products. Enzyme sequentially adds glycosyl residues with beta-1,6 connectivity, the first glycosyltransferase activity to dioleoylglycerol being about 1 order of magnitude higher than the second to monoglucosyldioleoylglycerol
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additional information
?
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1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl]-sn-glycerol, i.e. diglucosyl diacylglycerol, is not further converted to triglucosyl diacylglycerol. Not accepted as substrates are UDP-galactose, UDP-mannose, GDP-mannose, UDP-xylose, and UDP-Nacetylglucosamine. Cholesterol, cholesterolglucoside and the fluorescent substrate 6-[[(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]sphingosine, i.e.NBD-ceramide, can alos serve as acceptors that are converted into more polar products
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additional information
?
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1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl]-sn-glycerol, i.e. diglucosyl diacylglycerol, is not further converted to triglucosyl diacylglycerol. Not accepted as substrates are UDP-galactose, UDP-mannose, GDP-mannose, UDP-xylose, and UDP-Nacetylglucosamine. Cholesterol, cholesterolglucoside and the fluorescent substrate 6-[[(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]sphingosine, i.e.NBD-ceramide, can alos serve as acceptors that are converted into more polar products
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?
additional information
?
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1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl]-sn-glycerol, i.e. diglucosyl diacylglycerol, is not further converted to triglucosyl diacylglycerol. Not accepted as substrates are UDP-galactose, UDP-mannose, GDP-mannose, UDP-xylose, and UDP-Nacetylglucosamine. Cholesterol, cholesterolglucoside and the fluorescent substrate 6-[[(N-7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]sphingosine, i.e.NBD-ceramide, can alos serve as acceptors that are converted into more polar products
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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-
?
UDP-alpha-D-glucose + 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->6)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
can only use UDP-glucose as sugar donor
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?
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Jorasch, P.; Warnecke, D.C.; Lindner, B.; Zahringer, U.; Heinz, E.
Novel processive and nonprocessive glycosyltransferases from Staphylococcus aureus and Arabidopsis thaliana synthesize glycoglycerolipids, glycophospholipids, glycosphingolipids and glycosylsterols
Eur. J. Biochem.
267
3770-3783
2000
Staphylococcus aureus (Q5HH69), Staphylococcus aureus, Staphylococcus aureus COL (Q5HH69)
brenda
Jorasch, P.; Wolter, F.P.; Zahringer, U.; Heinz, E.
A UDP glucosyltransferase from Bacillus subtilis successively transfers up to four glucose residues to 1,2-diacylglycerol: expression of ypfP in Escherichia coli and structural analysis of its reaction products
Mol. Microbiol.
29
419-430
1998
Bacillus subtilis (P54166), Bacillus subtilis 60015 (P54166)
brenda
Andres, E.; Martinez, N.; Planas, A.
Expression and characterization of a Mycoplasma genitalium glycosyltransferase in membrane glycolipid biosynthesis: Potential target against Mycoplasma infections
J. Biol. Chem.
286
35367-35379
2011
Mycoplasma genitalium (Q9ZB73), Mycoplasma genitalium
brenda
Reichmann, N.T.; Picarra Cassona, C.; Monteiro, J.M.; Bottomley, A.L.; Corrigan, R.M.; Foster, S.J.; Pinho, M.G.; Gruendling, A.
Differential localization of LTA synthesis proteins and their interaction with the cell division machinery in Staphylococcus aureus
Mol. Microbiol.
92
273-286
2014
Staphylococcus aureus, Staphylococcus aureus Newman
brenda
2.4.1.315 diglucosyl diacylglycerol synthase (1,6-linking)
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