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1-O-4-coumaroyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
4-coumarate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-4-hydroxybenzoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
4-hydroxybenzoate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-caffeoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
caffeate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-feruloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
ferulate + cyanidin 3,5-di-O-beta-D-glucoside
-
1-O-feruloyl-beta-D-glucose is the best donor substrate
-
-
?
1-O-isovanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
isovanillate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-sinapoyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
sinapate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-(6-O-malyl)beta-D-glucoside
vanillate + cyanidin 3-O-(6-O-malyl)beta-D-glucosyl-5-O-beta-D-glucoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-galactoside
vanillate + cyanidin 3,5-di-O-beta-D-galactoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
vanillate + cyanidin 3,5-di-O-beta-D-glucoside
1-O-vanilloyl-beta-D-glucose + delphinidin 3-O-beta-D-glucoside
vanillate + delphinidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-(6-O-malyl)-beta-D-glucoside
vanillate + pelargonidin 3-O-(6-O-malyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + pelargonidin 3-O-beta-D-glucoside
vanillate + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
additional information
?
-
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
vanillate + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
-
?
1-O-vanilloyl-beta-D-glucose + cyanidin 3-O-beta-D-glucoside
vanillate + cyanidin 3,5-di-O-beta-D-glucoside
-
no activity with 1-O-vanilloyl-alpha-D-glucose
-
-
?
additional information
?
-
AB646511
AA5GT utilizes acyl-glucoses, but not UDP-glucose, as the glucose donor
-
-
?
additional information
?
-
-
AA5GT utilizes acyl-glucoses, but not UDP-glucose, as the glucose donor
-
-
?
additional information
?
-
-
substrate specificity, overview. AA7GT uses various acyl-glucose molecules, including benzoyl-glucose derivatives (C6-C1-glucoses) and 1-O-beta-hydroxycinnamoyl-glucoses (HCAGs, C6-C3-glucoses) but not flavonoid glucosides (C6-C3-C6-glucoses), as donor substrates, but it shows a strict acceptor preference for anthocyanidin 3-glucoside, 3-galactoside, and 3-malylglucoside and shows no preferences for other flavonoids or phenolic compounds. No activity with 1-O-beta-D-vanillyl-galactose, 4-nitropheny-glucoside, arbutin, salicin, populin, eleutheroside B, phloridzin, glucovanillin, esculin, kaempferol 3-glucoside, kaempferol 7-neohesperidoside, apigenin 7-glucoside, naringenin 7-glucoside, daidzin, indican. And no activity with kaempferol, kaempferol 3-glucoside, apigenin, apigenin 7-glucoside, naringenin, naringenin 7-glucoside, cyanidin, cyanidin 3,5-glucoside, cyanidin 3,7-glucosside, pelargonidin, delphinidin, (+) catechin, (-) epicatechin, 1-naphtol, 2-naphtol, NAA, indole acetic acid, 7-hydroxy-4-methylcoumarin, 7-hydroxy-6-methoxycoumarin, salicylic acid, phloroglucin
-
-
?
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0.0465 - 0.0519
1-O-vanilloyl-beta-D-glucose
0.0065 - 0.013
cyanidin 3-O-beta-D-glucoside
0.0465
1-O-vanilloyl-beta-D-glucose
-
pH 5.0, 30°C, native enzyme
0.0519
1-O-vanilloyl-beta-D-glucose
-
pH 5.0, 30°C, recombinant enzyme
0.0065
cyanidin 3-O-beta-D-glucoside
-
pH 5.0, 30°C, recombinant enzyme
0.013
cyanidin 3-O-beta-D-glucoside
-
pH 5.0, 30°C, native enzyme
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Matsuba, Y.; Sasaki, N.; Tera, M.; Okamura, M.; Abe, Y.; Okamoto, E.; Nakamura, H.; Funabashi, H.; Takatsu, M.; Saito, M.; Matsuoka, H.; Nagasawa, K.; Ozeki, Y.
A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium
Plant Cell
22
3374-3389
2010
Dianthus caryophyllus
brenda
Nishizaki, Y.; Matsuba, Y.; Okamoto, E.; Okamura, M.; Ozeki, Y.; Sasaki, N.
Structure of the acyl-glucose-dependent anthocyanin 5-O-glucosyltransferase gene in carnations and its disruption by transposable elements in some varieties
Mol. Genet. Genomics
286
383-394
2011
Dianthus caryophyllus (AB646511), Dianthus caryophyllus
brenda