One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
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The enzyme appears in viruses and cellular organisms
UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside
one of three specific glucosyltransferases in pea, Pisum sativum, that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside, see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase, TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly
One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.
the enzyme of cultivar Nezumisaya shows a broad activity for kaempferol/quercetin 3-O-glucoside/galactoside derivatives but does not glucosylate kaempferol 3-O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)-glucoside] and 3-O-rhamnosyl-(1->4)-[glucosyl-(1->6)-glucoside]
the enzyme of cultivar Nezumisaya shows a broad activity for kaempferol/quercetin 3-O-glucoside/galactoside derivatives but does not glucosylate kaempferol 3-O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)-glucoside] and 3-O-rhamnosyl-(1->4)-[glucosyl-(1->6)-glucoside]
glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities
no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol diglucoside, quercetin-3-rutinoside, quercetin-3-rhamnoside
glycosyltransferases are classified into at least 96 families, the family 1 glycosyltransferase, referred to as UDP glycosyltransferases (UGTs), comprise the largest group in plants. UGTs catalyze the transfer of a glycosyl moiety from UDP sugars to a wide range of acceptor molecules including flavonoids
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Fg3 or GmF3G2''Gt in the molecular linkage group C2, chromosome 6, DNA and amino acid sequence determination and analysis, genotyping, unrooted molecular phylogenetic tree