BRENDA Home
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB CommentsThe enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species.
The expected taxonomic range for this enzyme is: Brucella
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
wbkC
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + GDP-alpha-D-perosamine = tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate:GDP-alpha-D-perosamine N-formyltransferase
The enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + GDP-3-deoxyperosamine
tetrahydrofolate + GDP-N-formyl-3-deoxyperosamine
-
-
-
?
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
-
-
-
?
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
-
-
-
?
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GDP-alpha-D-perosamine
substrate inhibition by GDP-alpha-D-perosamine is evident above 0.25 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.46
GDP-alpha-D-perosamine
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.54
GDP-alpha-D-perosamine
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.2
GDP-alpha-D-perosamine
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
the enzyme is absolutely required for the O-side-chain production of the lipopolysaccharide O-antigen
physiological function
-
the enzyme is absolutely required for the O-side-chain production of the lipopolysaccharide O-antigen
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
WBKC_BRUA2
Brucella abortus (strain 2308)
259
0
29128
Swiss-Prot
-
WBKC_BRUME
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
259
0
29128
Swiss-Prot
-
Q9ZHX0_BRUML
259
0
29128
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 29100, calculated from amino acid sequence
?
-
x * 29100, calculated from amino acid sequence
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
mutant enzyme D78A/F142A, hanging drop vapor diffusion method, using 13-15% (w/v) poly(ethylene glycol) 5000, 2% (v/v) glycerol. Wild type enzyme, hanging drop vapor diffusion method, using 12-15% (w/v) poly(ethylene glycol) 8000m and 2% (w/v) 2-methyl-2,4-pentanediol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D78A/F142A
the mutant variant does not exhibit substrate inhibition, but does display kinetic behavior expected for an enzyme with modest cooperativity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-NTA resin column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli Rosetta (DE3) cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Riegert, A.S.; Chantigian, D.P.; Thoden, J.B.; Tipton, P.A.; Holden, H.M.
Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis
Biochemistry
56
3657-3668
2017
Brucella melitensis (Q9ZHX0)
brenda
Godfroid, F.; Cloeckaert, A.; Taminiau, B.; Danese, I.; Tibor, A.; De Bolle, X.; Mertens, P.; Letesson, J.
Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk)
Res. Microbiol.
151
655-668
2000
Brucella melitensis (Q9ZHX0), Brucella melitensis 16M (Q9ZHX0)
brenda
Select items on the left to see more content.
html completed