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Synonyms
gppmt, c-methyl transferase, sco7701, gpp methyltransferase, sco7701 protein, geranyl pyrophosphate methyltransferase, geranyl diphosphate c-methyltransferase, geranyl diphosphate 2-methyltransferase,
more
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
catalytic reaction mechanism, detailed overview
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
catalytic reaction mechanism, detailed overview
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
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S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
biosynthetic mechanism, overview
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S-adenosyl-L-methionine + (E)-2-methylgeranyl diphosphate
S-adenosyl-L-homocysteine + 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
additional information
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of the products from recombinant SCO7700 reveals the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene at 6%, beta-pinene at 23%, limonene at 32%, gamma-terpinene at 29%, and delta-terpinene at 10% accompanied by traces of the monoterpene alcohols beta-terpineol, 4-terpineol, and alpha-terpineol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
methylation of geranyl diphosphate before the cyclization reaction
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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GC-MS analysis of products
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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additional information
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SCO7701 shows no activity with with farnesyl diphosphate or geranylgeranyl diphosphate
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additional information
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SCO7701 shows no activity with with farnesyl diphosphate or geranylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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?
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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?
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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?
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
methylation of geranyl diphosphate before the cyclization reaction
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?
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
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S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
the enzyme is involved in the biosynthesis of the terpenoid alcohol 2-methylisoborneol
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?
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metabolism
geranyl diphosphate C-methyltransferase is the methyltransferase that modifies an acyclic isoprenoid diphosphate, geranyl diphosphate, to yield a noncanonical acyclic allylic diphosphate product, 2-methylgeranyl diphosphate, which serves as the substrate for a subsequent cyclization reaction catalyzed by a terpenoid cyclase, methylisoborneol synthase
metabolism
the C-methyl transferase SCO7701 catalyzes the two-step conversion of geranyl diphosphate to 2-methyl-isoborneol by way of 2-methylgeranyl diphosphate
metabolism
the enzyme catalyzes the first step in the biosynthesis of 2-methylisoborneol, overview
metabolism
the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
metabolism
the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
metabolism
the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
metabolism
the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
metabolism
some filamentous cyanobacteria produce 2-methylborneol via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). Pseudanabaena galeata, the filamentous cyanobacterium known to be a major causal organism of 2-methylborneol production in Japanese lakes. Metabolite analysis shows dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures
metabolism
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geranyl diphosphate C-methyltransferase is the methyltransferase that modifies an acyclic isoprenoid diphosphate, geranyl diphosphate, to yield a noncanonical acyclic allylic diphosphate product, 2-methylgeranyl diphosphate, which serves as the substrate for a subsequent cyclization reaction catalyzed by a terpenoid cyclase, methylisoborneol synthase
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metabolism
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the enzyme catalyzes the first step in the biosynthesis of 2-methylisoborneol, overview
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metabolism
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the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
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metabolism
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the enzyme is important in the biosynthesis of 2-methylisoborneol, which involves the methylation of geranyl diphosphate by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-methylisoborneol
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physiological function
geranyl diphosphate C-methyltransferase is the methyltransferase that modifies an acyclic isoprenoid diphosphate, geranyl diphosphate, to yield a noncanonical acyclic allylic diphosphate product, 2-methylgeranyl diphosphate, which serves as the substrate for a subsequent cyclization reaction catalyzed by a terpenoid cyclase, methylisoborneol synthase
physiological function
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geranyl diphosphate C-methyltransferase is the methyltransferase that modifies an acyclic isoprenoid diphosphate, geranyl diphosphate, to yield a noncanonical acyclic allylic diphosphate product, 2-methylgeranyl diphosphate, which serves as the substrate for a subsequent cyclization reaction catalyzed by a terpenoid cyclase, methylisoborneol synthase
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purified recombinant His6-tagged wild-type enzyme in complex with S-adenosyl-L-methionine and monoterpenoid ligands geranyl-S-thiolodiphosphate, a substrate analogue, or geranyl diphosphate, sitting drop vapor diffusion method, 4°C, mixing of 0.001 ml protein solution conatining 6 mg/mL enzyme, 50 mM PIPES, pH 6.7, 20% glycerol, 5 mM BME, 15 mM MgCl2, 100 mM NaCl, 10 mM S-adenosyl-L-methionine, and 1 mM monoterpenoid ligand with 0.001 ml of precipitant solution containing 100 mM HEPES, pH 7.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl-S-thiolodiphosphate, 100 mM Bis-Tris, pH 6.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl diphosphate and equilibrated against a 0.1 ml reservoir of precipitant solution at 21°C, 1 day, X-ray diffraction structure determination and analysis at 2.05 A resolution
purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4°C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method
sitting-drop vapour-diffusion method
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as N-terminal His6-tagged protein
expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene pgmtf, real-time-RT PCR enzyme expression analysis
gene sco7700, DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
overproduced in Escherichia coli strain BL21
wild-type and mutant geranyl diphosphate methyltransferase expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as N-terminal His6-tagged protein
expression in Escherichia coli as N-terminal His6-tagged protein
expression in Escherichia coli as N-terminal His6-tagged protein
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Ariyawutthiphan, O.; Ose, T.; Tsuda, M.; Gao, Y.; Yao, M.; Minami, A.; Oikawa, H.; Tanaka, I.
Crystallization and preliminary X-ray crystallographic study of a methyltransferase involved in 2-methylisoborneol biosynthesis in Streptomyces lasaliensis
Acta Crystallogr. Sect. F
67
417-420
2011
Streptomyces lasalocidi (D3KYU3), Streptomyces lasalocidi
brenda
Giglio, S.; Chou, W.K.; Ikeda, H.; Cane, D.E.; Monis, P.T.
Biosynthesis of 2-methylisoborneol in cyanobacteria
Environ. Sci. Technol.
45
992-998
2010
Pseudanabaena limnetica (E9NH27), Pseudanabaena limnetica, Oscillatoria limosa (E9NH31), Pseudanabaena sp. (E9NH33), Pseudanabaena limnetica Castaic Lake (E9NH27), Pseudanabaena sp. NIVA-CYA 111 (E9NH33), Oscillatoria limosa LBD 305b (E9NH31)
brenda
Wang, C.M.; Cane, D.E.
Biochemistry and molecular genetics of the biosynthesis of the earthy odorant methylisoborneol in Streptomyces coelicolor
J. Am. Chem. Soc.
130
8908-8909
2008
Streptomyces coelicolor (Q9F1Y5), Streptomyces coelicolor
brenda
Wang, Z.; Xu, Y.; Shao, J.; Wang, J.; Li, R.
Genes associated with 2-methylisoborneol biosynthesis in cyanobacteria: isolation, characterization, and expression in response to light
PLoS One
6
e18665
2011
Pseudanabaena sp. (G0YUP0), Planktothricoides raciborskii (G0YUP4), Planktothricoides raciborskii CHAB3331 (G0YUP4)
brenda
Komatsu, M.; Tsuda, M.; Omura, S.; Oikawa. H.; Ikeda, H.
Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol
Proc. Natl. Acad. Sci. USA
105
7422-7427
2008
Streptomyces ambofaciens (A3KI18), Saccharopolyspora erythraea (A4FG18), Saccharopolyspora erythraea, Streptomyces lasalocidi (D3KYU3), Streptomyces lasalocidi, Micromonospora olivasterospora (F5HSX5), Micromonospora olivasterospora, Streptomyces lasalocidi NRRL2382R (D3KYU3), Micromonospora olivasterospora KY11048 (F5HSX5), Micromonospora olivasterospora KY11048, Saccharopolyspora erythraea NRRL 2338 (A4FG18), Saccharopolyspora erythraea NRRL 2338, Streptomyces ambofaciens ISP5053 (A3KI18)
brenda
Koeksal, M.; Chou, W.K.; Cane, D.E.; Christianson, D.W.
Structure of geranyl diphosphate C-methyltransferase from Streptomyces coelicolor and implications for the mechanism of isoprenoid modification
Biochemistry
51
3003-3010
2012
Streptomyces coelicolor (Q9F1Y5), Streptomyces coelicolor, Streptomyces coelicolor A3(2) (Q9F1Y5), Streptomyces coelicolor A3(2)
brenda
Kakimoto, M.; Ishikawa, T.; Miyagi, A.; Saito, K.; Miyazaki, M.; Asaeda, T.; Yamaguchi, M.; Uchimiya, H.; Kawai-Yamada, M.
Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata
J. Plant Physiol.
171
292-300
2014
Pseudanabaena galeata (U6C870), Pseudanabaena galeata
brenda