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Information on EC 1.8.3.3 - glutathione oxidase
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1.8.3.3 glutathione oxidaseIUBMB Comments
A flavoprotein (FAD). Also acts, more slowly, on L-cysteine and several other thiols.
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Word Map
- 1.8.3.3
- fad
- oxidases
- transpeptidase
-
gamma-glutamyl
- gamma-glutamyltransferase
- analysis
- medicine
The enzyme appears in viruses and cellular organisms
Synonyms
glutathione oxidase, flavin-linked sulfhydryl oxidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glutathione sulfhydryl oxidase
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-
-
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GSH oxidase >
-
-
-
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GSH sulfhydryl oxidase
-
-
-
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oxidase, glutathione
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-
-
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Sox1
sulfhydryl oxidase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 glutathione + O2 = glutathione disulfide + H2O2
a FAD flavoprotein, acts, more slowly, also on L-cysteine and several other thiols
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
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reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
glutathione:oxygen oxidoreductase
A flavoprotein (FAD). Also acts, more slowly, on L-cysteine and several other thiols.
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CAS REGISTRY NUMBER
COMMENTARY
55467-56-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dithiothreitol + cytochrome c
dithiothreitol disulfide + reduced cytochrome c
-
-
-
-
?
S-methylglutathione + O2
?
-
enzyme immobilized on microelectrode sensor, low activity
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-
?
2-mercaptoethanol + O2
mercaptoethanol disulfide
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19% of the activity with glutathione
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-
?
glutathione + O2
glutathione disulfide + H2O2
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enzyme immobilized on microsensor
-
?
glutathione + O2
glutathione disulfide + H2O2
-
enzyme immobilized on microsensor
-
?
glutathione + O2
glutathione disulfide + H2O2
-
measurement of H2O2 in vivo via microelectrodes with immobilized enzyme
-
?
glutathione + O2
glutathione disulfide + H2O2
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measurement of glutathione in vivo via microelectrodes with immobilized enzyme
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?
additional information
?
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ALT-2074, a catalytic mimic of glutathione oxidase, inhibits human cytochrome P450 3A isoforms in vitro
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-
?
additional information
?
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no other amio acids but D- and L-cysteine serve as substrate
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-
?
additional information
?
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D-penicilamine and coenzyme A are no substrates
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-
?
additional information
?
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Penicillium sp. K-6-5
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no other amio acids but D- and L-cysteine serve as substrate
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glutathione + O2
glutathione disulfide + H2O2
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measurement of H2O2 in vivo via microelectrodes with immobilized enzyme
-
?
glutathione + O2
glutathione disulfide + H2O2
-
measurement of glutathione in vivo via microelectrodes with immobilized enzyme
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Neoplasms
Biocompatible Ruthenium Single-Atom Catalyst for Cascade Enzyme-Mimicking Therapy.
Neoplasms
Self-Assembled Single-Site Nanozyme for Tumor-Specific Amplified Cascade Enzymatic Therapy.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.7
glutathione
-
immobilized enzyme on sensor surface out of cellulose acetate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3.8 - 11
-
enzyme immobilized on microsensor surface out of cellulose acetate
5 - 10
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q2UA33_ASPOR
Aspergillus oryzae (strain ATCC 42149 / RIB 40)
384
0
42498
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
monomer
dimer
-
2 * 14000, SDS-PAGE, after centrifugation of infected cell extracts in glycerol gradients
dimer
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disulfide-bridged dimer (linked via C15-C124) with two free cysteine residues (C74 and 85) per monomer
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
glycoprotein
sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da
glycoprotein
-
sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after 36 measurements with enzyme immobilized on microelectrode sensor loss of 30% activity
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after 50 measurements with enzyme immobilized on microelectrode sensor loss of 50% activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 6.5, phosphate buffer, immobilized enzyme on microelectrode sensor, loss of 20% activity, 60 days
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4°C, phosphate buffered saline, immobilized enzyme on microelectrode sensor, loss of 20% activity, 68 days
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
medicine
-
ALT-2074, a catalytic mimic of glutathione oxidase, inhibits human cytochrome P450 3A isoforms in vitro
analysis
-
development of amperometric glutathione electrodes with immobilized enzyme for detection of H2O2
analysis
-
development of a glutathione micro-enzyme sensor for amperometric detection in vivo, immobilization of enzyme on microelectrode tips
analysis
-
development of an amperometric biosensor for determination of glutathione, by immobilizing covalently a glutathione oxidase onto the surface of gold coated magnetic nanoparticles modified Pt electrode. The electrode shows maximum response within 4 s, when polarizes at +0.4 V, pH 7.0 and 25°C. There is a linear relationship between electrode response and glutathione concentrations in the range 5.0-4000 microM with a detection limit of 0.1 microM. The biosensor shows 50% loss in its initial activity after its 150 uses over a period 4 months. Glutathione concentration in hemolysated erythrocytes as measured by the present biosensor is 2.8 mM in apparently healthy persons
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kusakabe, H.; Midorikawa, Y.; Kuninaka, A.; Yoshino, H.
Distribution of extracellular oxygen related enzymes in molds
Agric. Biol. Chem.
47
1385-1387
1983
Aspergillus sp., Penicillium sp., Fusarium sp., no activity in Mucor sp., no activity in Rhizopus sp.
-
brenda
Ostrowski, M.C.; Kistler, W.S.
Properties of a flavoprotein sulfhydryl oxidase from rat seminal vesicle secretion
Biochemistry
19
2639-2645
1980
Rattus norvegicus
brenda
Kusakaba, H.; Kuninaka, A.; Yoshino, H.
Purification and properties of a new enzyme, glutathione oxidase from Penicillium sp. K-6-5
Agric. Biol. Chem.
46
2057-2067
1982
Penicillium sp., Penicillium sp. K-6-5
-
brenda
Huang, Z.; Villarta-Snow, R.L.; Lubrano, G.J.; Guilbault, G.G.
Glutathione amperometric enzyme microsensor
Anal. Lett.
27
263-271
1994
Penicillium sp.
-
brenda
Compagnone, D.; Federici, G.; Scarciglia, L.; Palleschi, G.
Amperometric glutathione electrodes
Biosens. Bioelectron.
8
257-263
1993
Penicillium sp.
-
brenda
Farrell, S.R.; Thorpe, C.
Augmenter of liver regeneration: a flavin-dependent sulfhydryl oxidase with cytochrome c reductase activity
Biochemistry
44
1532-1541
2005
Homo sapiens
brenda
Rodriguez, I.; Redrejo-Rodriguez, M.; Rodriguez, J.M.; Alejo, A.; Salas, J.; Salas, M.L.
African swine fever virus pB119L protein is a flavin adenine dinucleotide-linked sulfhydryl oxidase
J. Virol.
80
3157-3166
2006
African swine fever virus
brenda
Greenblatt, D.J.; Peters, D.E.; Oleson, L.E.; Harmatz, J.S.; MacNab, M.W.; Berkowitz, N.; Zinny, M.A.; Court, M.H.
Inhibition of oral midazolam clearance by boosting doses of ritonavir, and by 4,4-dimethyl-benziso-(2H)-selenazine (ALT-2074), an experimental catalytic mimic of glutathione oxidase
Br. J. Clin. Pharmacol.
68
920-927
2009
Homo sapiens
brenda
Faccio, G.; Kruus, K.; Buchert, J.; Saloheimo, M.
Secreted fungal sulfhydryl oxidases: Sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae
BMC Biochem.
11
31
2010
Aspergillus oryzae (Q2UA33), Aspergillus oryzae ATCC 42149 (Q2UA33)
brenda
Chauhan, N.; Narang, J.; Meena, J.; Pundir, C.S.
An amperometric glutathione biosensor based on chitosan-iron coated gold nanoparticles modified Pt electrode
Int. J. Biol. Macromol.
51
879-886
2012
unidentified
brenda
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