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L-arogenate + NADP+
L-tyrosine + NADPH + H+ + CO2
Methanothrix harundinacea
no substrate of wild-type, reaction of EC 1.3.1.78
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH + H+
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + NADPH + H+ + CO2
Methanothrix harundinacea
-
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
dual cofactor specificity with a preference for NADP+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
the enzyme prefers NADP+ over NAD+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
Lyngbya sp.
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
the enzyme prefers NADP+ over NAD+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
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prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
-
?
prephenate + NAD+
4-hydroxyphenylpyruvate + CO2 + NADH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
dual cofactor specificity with a preference for NADP+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
the enzyme prefers NADP+ over NAD+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
Lyngbya sp.
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
the enzyme prefers NADP+ over NAD+
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
prephenate + NADP+
4-hydroxyphenylpyruvate + CO2 + NADPH
-
-
-
-
?
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3.29
L-arogenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
0.561
NAD+
at pH 8.0 and 80°C
3.3
NAD+
-
at pH 8.0 and 80°C
0.005
NADP+
-
-
0.012
NADP+
-
pretyrosine as substrate
0.014
NADP+
-
prephenate as substrate
0.062
NADP+
at pH 8.0 and 80°C
0.36
NADP+
-
at pH 8.0 and 80°C
0.033
prephenate
-
-
0.127
prephenate
-
at 37°C, pH not specified in the publication
0.14
prephenate
-
mutant T206S, pH 7.5, 37°C
0.17
prephenate
-
mutant T131S, pH 7.5, 37°C
0.21
prephenate
-
mutant T131A, pH 7.5, 37°C
0.22
prephenate
-
mutant Q130A, pH 7.5, 37°C
0.223
prephenate
-
at 37°C, pH not specified in the publication
0.26
prephenate
-
mutant Q130S, pH 7.5, 37°C
0.27
prephenate
-
wild-type, pH 7.5, 37°C
0.29
prephenate
-
mutant H124N, pH 7.5, 37°C
0.319
prephenate
at pH 8.0 and 80°C
0.319
prephenate
with NAD+ as cosubstrate, at pH 8.0 and 80°C
0.37
prephenate
-
mutant Q184A, pH 7.5, 37°C
0.38
prephenate
Methanothrix harundinacea
wild-type, pH 7.6, temperature not specified in the publication
0.44
prephenate
-
mutant Q130E, pH 7.5, 37°C
0.443
prephenate
with NADP+ as cosubstrate, at pH 8.0 and 80°C
0.48
prephenate
-
mutant Q130D, pH 7.5, 37°C
0.49
prephenate
-
mutant N222Q, pH 7.5, 37°C
0.69
prephenate
-
with NAD+ as cosubstrate, at pH 8.0 and 80°C
0.73
prephenate
-
with NADP+ as cosubstrate, at pH 8.0 and 80°C
2.67
prephenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
5.2
prephenate
-
mutant S101A, pH 7.5, 37°C
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0.7
L-arogenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
0.018
prephenate
-
mutant T206S, pH 7.5, 37°C
0.052
prephenate
-
mutant Q184A, pH 7.5, 37°C
0.055
prephenate
-
mutant Q130A, pH 7.5, 37°C
0.06
prephenate
-
mutant T131S, pH 7.5, 37°C
0.075
prephenate
-
mutant H124N, pH 7.5, 37°C
0.122
prephenate
-
mutant T131A, pH 7.5, 37°C
0.29
prephenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
0.5
prephenate
-
mutant N222Q, pH 7.5, 37°C
0.583
prephenate
-
mutant Q130S, pH 7.5, 37°C
2.44
prephenate
Methanothrix harundinacea
wild-type, pH 7.6, temperature not specified in the publication
2.83
prephenate
-
mutant Q130D, pH 7.5, 37°C
3.83
prephenate
-
mutant Q130E, pH 7.5, 37°C
15
prephenate
at pH 8.0 and 80°C
16.3
prephenate
-
wild-type, pH 7.5, 37°C
18.3
prephenate
-
mutant S101A, pH 7.5, 37°C
21.8
prephenate
-
at 37°C, pH not specified in the publication
26.3
prephenate
-
at 37°C, pH not specified in the publication
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0.21
L-arogenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
0.015
prephenate
-
mutant H124N, pH 7.5, 37°C
0.11
prephenate
Methanothrix harundinacea
mutant Q227E, pH 7.6, temperature not specified in the publication
0.13
prephenate
-
mutant T206S, pH 7.5, 37°C
0.14
prephenate
-
mutant Q184A, pH 7.5, 37°C
0.25
prephenate
-
mutant Q130A, pH 7.5, 37°C
0.36
prephenate
-
mutant T131S, pH 7.5, 37°C
0.58
prephenate
-
mutant T131A, pH 7.5, 37°C
1.02
prephenate
-
mutant N222Q, pH 7.5, 37°C
2.22
prephenate
-
mutant Q130S, pH 7.5, 37°C
3.53
prephenate
-
mutant S101A, pH 7.5, 37°C
5.9
prephenate
-
mutant Q130D, pH 7.5, 37°C
6.44
prephenate
Methanothrix harundinacea
wild-type, pH 7.6, temperature not specified in the publication
8.71
prephenate
-
mutant Q130E, pH 7.5, 37°C
47
prephenate
at pH 8.0 and 80°C
60.5
prephenate
-
wild-type, pH 7.5, 37°C
100
prephenate
-
at 37°C, pH not specified in the publication
217
prephenate
-
at 37°C, pH not specified in the publication
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ATCC 15309
-
-
brenda
-
-
-
brenda
-
-
-
brenda
strain ATCC 27893, 27898, 29151 and 29413
-
-
brenda
-
-
-
brenda
-
-
-
brenda
strain ATCC 29538
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
Lyngbya sp.
strain ATCC 29119, 29121 and 29126
-
-
brenda
Methanothrix harundinacea
-
UniProt
brenda
-
-
-
brenda
no activity in Anabaena sp.
strains ATCC 27892, 27899, 29211 and 29414
-
-
brenda
no activity in Arabidopsis thaliana
-
-
-
brenda
no activity in Beta vulgaris subsp. vulgaris
red beet, Beta vulgaris ssp. var. conditiva, Beta vulgaris ssp. vulgaris var. rubra
-
-
brenda
no activity in Calothrix sp.
strain ATCC 27901, 27905, 29112, 29156, 29158and 29171
-
-
brenda
no activity in Nostoc sp.
strain ATCC 29411 and 29132
-
-
brenda
no activity in Oryza sativa
rice
-
-
brenda
no activity in Pisum sativum
pea
-
-
brenda
no activity in Scytonema sp.
-
-
-
brenda
no activity in Scytonema sp. 29171
-
-
-
brenda
no activity in Synechocystis sp.
strain ATCC 29108
-
-
brenda
no activity in Triticum aestivum
wheat
-
-
brenda
strain ATCC 29105 and 29133
-
-
brenda
scarlet runner bean, L.
-
-
brenda
wax bean, L. var.Bountiful
-
-
brenda
Pseudomonas alkanolytica
-
-
-
brenda
strain ATCC 29404, Agmenellum quadruplicatum BG1, strain 27144, 27146 and 27192
-
-
brenda
strain ATCC 29109,27178 and 29235
-
-
brenda
broad bean, L. var. Windsor
-
-
brenda
mung bean, [L.] Wilczek, originally classified as Phaseolus aureus [L.] Roxb.
-
-
brenda
ATCC 14987
-
-
brenda
ATCC 23055
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
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H124N
-
substantial decrease in catalytic efficiency
N222A
-
complete loss of activity
N222Q
-
30fold decrease in kcat value and a modest change in Km value
Q130A
-
300fold lower turnover rate
Q130D
-
4fold reduction in kcat values and less than twofold increase in Km value
Q130E
-
4fold reduction in kcat values and less than twofold increase in Km value
Q130S
-
35fold lower turnover rate
Q184A
-
320fold lower kcat value, with a Km value comparable to that of the wild-type
Q184D
-
loss of detectable PDH activity
Q184E
-
loss of detectable PDH activity
S101A
-
mutantion primarily affectes the Km value for prephenate with a 20fold increase
T131A
-
134fold decrease in kcat value
T131S
-
272fold decrease in kcat value
T206A
-
loss of detectable activity
T206S
-
tremendous loss of activity
Q227E
Methanothrix harundinacea
mutation introduces arogenate dehydrogenase activity
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Waldner-Sander, S.; Keller, B.; Keller, E.; Lingens, F.
Zur Biosynthese von Phenylalanin und Tyrosin bei Flavobakterien
Hoppe-Seyler's Z. Physiol. Chem.
364
1467-1473
1983
Flavobacterium sp.
brenda
Gamborg, O.L.; Keeley, F.W.
Aromatic metabolism in plants I. A study of the prephenate dehydrogenase from bean plants
Biochim. Biophys. Acta
115
65-72
1966
Klebsiella aerogenes, Vicia faba, no activity in Beta vulgaris subsp. vulgaris, no activity in Oryza sativa, no activity in Pisum sativum, no activity in Triticum aestivum, Phaseolus coccineus, Vigna radiata, Phaseolus vulgaris
brenda
Rubin, J.L.; Jensen, R.A.
Enzymology of L-tyrosine biosynthesis in mung bean (Vigna radiata [L.] Wilczek)
Plant Physiol.
64
727-734
1979
Burkholderia cepacia, Comamonas testosteroni, Vigna radiata, Pseudomonas alkanolytica
brenda
Hall, G.C.; Flick, M.B.; Gherna, R.L.; Jensen, R.A.
Biochemical diversity for biosynthesis of aromatic amino acids among the cyanobacteria
J. Bacteriol.
149
65-78
1982
Anabaena sp., Synechocystis sp., Fischerella sp., Lyngbya sp., no activity in Anabaena sp., no activity in Calothrix sp., no activity in Nostoc sp., no activity in Scytonema sp., no activity in Synechocystis sp., Nostoc sp., Synechococcus sp., no activity in Scytonema sp. 29171
brenda
Byng, G.S.; Berry, A.; Jensen, R.A.
Evolutionary implications of features of aromatic amino acid biosynthesis in the genus Acinetobacter
Arch. Microbiol.
143
122-129
1985
Acinetobacter calcoaceticus, Acinetobacter lwoffii, Acinetobacter sp., Acinetobacter sp. C1W
brenda
Shlaifer, I.; Quashie, P.K.; Kim, H.Y.; Turnbull, J.L.
Biochemical characterization of TyrA enzymes from Ignicoccus hospitalis and Haemophilus influenzae: A comparative study of the bifunctional and monofunctional dehydrogenase forms
Biochim. Biophys. Acta
1865
312-320
2017
Ignicoccus hospitalis (A8AAX2)
brenda
Shlaifer, I.; Turnbull, J.L.
Characterization of two key enzymes for aromatic amino acid biosynthesis in symbiotic archaea
Extremophiles
20
503-514
2016
Nanoarchaeum equitans, Ignicoccus hospitalis (A8AAX2), Ignicoccus hospitalis
brenda
Schenck, C.A.; Chen, S.; Siehl, D.L.; Maeda, H.A.
Non-plastidic, tyrosine-insensitive prephenate dehydrogenases from legumes
Nat. Chem. Biol.
11
52-57
2015
Glycine max, Medicago truncatula, no activity in Arabidopsis thaliana
brenda
Holland, C.; Jez, J.
Reaction mechanism of prephenate dehydrogenase from the alternative tyrosine biosynthesis pathway in plants
ChemBioChem
19
1132-1136
2018
Glycine max
brenda
Schenck, C.; Men, Y.; Maeda, H.
Conserved molecular mechanism of TyrA dehydrogenase substrate specificity underlying alternative tyrosine biosynthetic pathways in plants and microbes
Front. Mol. Biosci.
4
73
2017
Methanothrix harundinacea (A0A101IGG2)
brenda
Schenck, C.; Westphal, J.; Jayaraman, D.; Garcia, K.; Wen, J.; Mysore, K.; Ane, J.; Sumner, L.; Maeda, H.
Role of cytosolic, tyrosine-insensitive prephenate dehydrogenase in Medicagoxa0truncatula
Plant Direct
4
e00218
2020
Medicago truncatula (G7J2E9), Medicago truncatula
brenda