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1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
4-androsten-3,17-dione + AH2 + O2
?
-
-
-
-
?
4-androstene-3,17-dione + NADH + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
progesterone + AH2 + O2
9alpha-hydroxyprogesterone + A + H2O
additional information
?
-
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
-
-
-
?
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system in steroid nucleus degradation in company with delta-dehydrogenase
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key-enzyme in the steroid-ring-B-splitting pathway
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key-enzyme in the steroid-ring-B-splitting pathway
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
-
-
?
4-androstene-3,17-dione + NADH + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
-
?
4-androstene-3,17-dione + NADH + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
-
?
4-androstene-3,17-dione + NADH + O2
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
9(11)-dehydro-17alpha-methyl-testosterone + NADH + O2
9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-4-androstene-3-one + 9alpha,11alpha-oxido-17beta-hydroxy-17alpha-methyl-1,4-androstadiene-3-one + NAD+ + H2O
-
-
-
-
?
progesterone + AH2 + O2
9alpha-hydroxyprogesterone + A + H2O
-
reaction with whole cells in a bioreactor
-
-
?
progesterone + AH2 + O2
9alpha-hydroxyprogesterone + A + H2O
-
reaction with whole cells in a bioreactor, low in vitro activity
-
-
?
additional information
?
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
additional information
?
-
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
additional information
?
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
additional information
?
-
-
product determination and identification by LLE, HPLC-DAD, MS, and NMR, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
progesterone + AH2 + O2
9alpha-hydroxyprogesterone + A + H2O
-
reaction with whole cells in a bioreactor
-
-
?
additional information
?
-
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
-
-
-
?
1,4-androstadiene-3,17-dione + 2 O2 + 2 H+
9-hydroxy-1,4-androstadiene-3,17-dione + H2O2
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system in steroid nucleus degradation in company with delta-dehydrogenase
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key-enzyme in the steroid-ring-B-splitting pathway
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key-enzyme in the steroid-ring-B-splitting pathway
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
-
-
?
additional information
?
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
additional information
?
-
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
additional information
?
-
KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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metabolism
-
3-ketosteroid 9alpha-hydroxylase is a critical pathogenicity factor of strainH37Rv involved in steroid and cholesterol catabolism
metabolism
key enzyme in steroid degradation. The two key enzymes, 3-ketosteroid DELTA1-dehydrogenase, KsdD, and 3-ketosteroid 9alpha-hydroxylase, KSH, are essential for the production and accumulation of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from soy bean, Glycine max
metabolism
-
key enzyme in steroid degradation. The two key enzymes, 3-ketosteroid DELTA1-dehydrogenase, KsdD, and 3-ketosteroid 9alpha-hydroxylase, KSH, are essential for the production and accumulation of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from soy bean, Glycine max
-
metabolism
-
3-ketosteroid 9alpha-hydroxylase is a critical pathogenicity factor of strainH37Rv involved in steroid and cholesterol catabolism
-
physiological function
-
genes kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis
physiological function
-
genes kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis
-
additional information
-
DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme. The deletion of either of these genes leads to rapid death of the microorganism in murine infection models and in macrophages, showing that kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis
additional information
-
DELTAkshA and DELTAkshB deletion mutants of strain H37Rv are unable to use cholesterol and 4-androstene-3,17-dione as primary carbon and energy sources, and are also unable to metabolize the steroid substrate 5alpha-androstane-3,17-dione in contrast to the wild-type enzyme. The deletion of either of these genes leads to rapid death of the microorganism in murine infection models and in macrophages, showing that kshA and kshB are essential factors for Mycobacterium tuberculosis pathogenesis
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Strijewski A.
The steroid-9alpha-hydroxylation system from Nocardia species
Eur. J. Biochem.
128
125-135
1982
Arthrobacter sp., Corynebacterium sp., Mycobacterium sp., Nocardia sp., Nocardia sp. M117
brenda
Sonomoto K.; Usuin N.; Tanaka A.; Fukui S.
9alpha-Hydroxylation of 4-androstene-3,17-dione by gel-entrapped Corynebacterium sp. cells
Eur. J. Appl. Microbiol. Biotechnol.
17
203-210
1983
Corynebacterium sp.
-
brenda
Kang, H.K.; Lee, S.S.
Heterogeneous natures of the microbial steroid 9alpha-hydroxylase in nocardioforms
Arch. Pharm. Res.
20
519-524
1997
Corynebacterium sp., Mycobacterium sp., Mycolicibacterium fortuitum, Nocardia sp., Rhodococcus erythropolis, Rhodococcus rhodochrous, Rhodococcus sp., Nocardia sp. M117, Rhodococcus rhodochrous KCTC 1061 / ATCC 17895, Rhodococcus erythropolis KCTC 1062
brenda
Kang, H.K.; Lee, S.S.
Microbial 9alpha-hydroxylase: epoxidation of 9(11)-dehydro-17a-methyl-testosterone
Arch. Pharm. Res.
20
525-528
1997
Corynebacterium sp.
brenda
Kang, H.K.; Lee, S.S.
Purification of the NADH reductase component of the steroid 9alpha-hydroxylase from Mycobacterium fortuitum
Arch. Pharm. Res.
20
590-596
1997
Mycolicibacterium fortuitum, Nocardia sp.
brenda
Mutafov, S.; Angelova, B.; Avramova, T.; Boyadjieva, L.; Dimova, I.
The inducibility of 9alpha-steroid hydroxylating activity in resting Rhodococcus sp. cells
Process Biotechnol.
32
585-589
1997
Corynebacterium sp., Rhodococcus sp., Rhodococcus sp. IOC-77
-
brenda
Arnell, R.; Johannisson, R.; Lindholm, J.; Fornstedt, T.; Ersson, B.; Ballagi, A.; Caldwell, K.
Biotechnological approach to the synthesis of 9alpha-hydroxylated steroids
Prep. Biochem. Biotechnol.
37
309-321
2007
Mycolicibacterium smegmatis
brenda
Wei, W.; Fan, S.; Wang, F.; Wei, D.
A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9alpha-hydroxylase in NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
Mycolicibacterium neoaurum (C9E9N6), Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01 (C9E9N6)
brenda
Hu, Y.; Van Der Geize, R.; Besra, G.; Gurcha, S.; Liu, A.; Rohde, M.; Singh, M.; Coates, A.
3-Ketosteroid 9alpha-hydroxylase is an essential factor in the pathogenesis of Mycobacterium tuberculosis
Mol. Microbiol.
75
107-121
2010
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda