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(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
(24R),25-dihydroxyvitamin D3 + NADPH + O2
1alpha,24,25-trihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
1alpha-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
20-hydroxyvitamin D3 + NADPH + O2
1alpha,20-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
23,25-dihydroxycholecalciferol + NADPH + O2
?
24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2
1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O
-
-
-
-
r
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2
?
24-oxo-25-hydroxycholecalciferol + NADPH + O2
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
25-hydroxyvitamin D2 + NADPH + H+ + O2
1,25-dihydroxyvitamin D2 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + H+ + O2
25-hydroxyvitamin D3 + H2O
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
1,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
cholecalciferol + NADPH + O2
25-hydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
additional information
?
-
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
higher activity than with 25-hydroxycholecalciferol
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
higher activity than with 25-hydroxycholecalciferol
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
reconstituted system
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
recombinant enzyme
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
higher activity than with 25-hydroxycholecalciferol
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
higher activity than with 25-hydroxycholecalciferol
-
?
23,25-dihydroxycholecalciferol + NADPH + O2
?
-
low activity
-
-
?
23,25-dihydroxycholecalciferol + NADPH + O2
?
-
recombinant enzyme
-
-
?
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2
?
-
low activity
-
-
?
24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2
?
-
recombinant enzyme
-
-
?
24-oxo-25-hydroxycholecalciferol + NADPH + O2
?
-
low activity, reconstituted system
-
-
?
24-oxo-25-hydroxycholecalciferol + NADPH + O2
?
-
recombinant enzyme
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
energy-dependent transhydrogenation is of importance
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
enzyme activity is correlated with phosphate content in serum
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
1,25(OH)2D3 is an antiproliferative agent that may inhibit proliferation of breast cancer cells
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
1,25-dihydroxyvitamin D3 (1,25-D3) is a key determinant of calcium homeostasis, cell proliferation and differentiation, calcium and vitamin D might have a protective function against colorectal tumor pathogenesis, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
bioactivation
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
extra-renal functions of 1alpha-hydroxylase in vitamin D physiology, regulation, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
regulation of enzyme expression in kidney proximal tubules, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
substrate activation by the rate-limiting enzyme 1alpha-hydroxylase in cells of the immune system under control of immune stimuli, such as interferon-gamma, in pathological situations, such as sarcoidosis, this can lead to systemic excess of 1,25(OH)2D3 and hypercalcemia
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
the enzyme hydroxylates the major circulating form of vitamin D to the active systemic hormone 1,25(OH)2D3, local production of 1,25(OH)2D3 appears to occur also at other sites where 1alpha-hydroxylase is expressed for autocrine/paracrine regulation, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
the enzyme is involved in the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate, mechanism, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
the enzyme is responsible for final hydroxylation of vitamin D3, in response to IFNgamma and CD14/TLR4 activation
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
deficiency of the enzyme, due to mutation of residues Q65 and T409, in substrate binding causes vitamin D-dependent rickets type 1, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
binding of 25-hydroxyvitamin D3 in a structural pocket involving residues Q65, S408, and T409, molecular modeling and substrate docking
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D, involved in tumor suppressing activity due to the tumor growth inhibiting effect of 1alpha,25-dihydroxyvitamin D3
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant enzyme
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
enzyme shows vitamin D3 25- and 1alpha-hydroxylase activity
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
-
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
calcidiol + NADPH + O2
calcitriol + NADP+ + H2O
highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol
calcitriol is identical with 1,25-dihydroxycholecalciferol
?
additional information
?
-
-
not: cholecalciferol, dihydrotachysterol
-
-
?
additional information
?
-
-
the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity
-
-
?
additional information
?
-
-
not active with (23S),25-dihydroxycholecalciferol and 24-oxo-23,25-dihydroxycholecalciferol
-
-
?
additional information
?
-
-
25(OH)D3 therapy is useful for colorectal cancer chemoprevention but provitamin D is less likely to be useful for colorectal cancer chemotherapy, overview
-
-
?
additional information
?
-
-
both butyrate and 1alpha-25(OH)2D3 stimulate differentiation of Caco-2 cells, while 25(OH)2D3 has no impact on cell differentiation
-
-
?
additional information
?
-
-
colonic and renal vitamin D hydroxylases are regulated independently
-
-
?
additional information
?
-
-
functional analysis of vitamin D metabolism in cancer, locally synthesized 1,25(OH)2D3 may be inefficient in preventing tumor progression, mechanism, overview
-
-
?
additional information
?
-
-
immune regulation of the enzyme in monocytes, cross-talk between the JAK-STAT, the NFkappaB, and the p38 MAPK pathways is necessary, and direct binding of C/EBPbeta to its recognition sites in the promoter of the 1alpha-hydroxylase gene is a prerequisite, overview
-
-
?
additional information
?
-
-
the enzyme is involved in local production of 1alpha,25-dihydroxyvitamin D, i.e. 1alpha,25(OH)2D3, in tumorigenesis
-
-
?
additional information
?
-
-
the 1alpha-OHase gene is a tumor suppressor gene responsible for the normal regulation of prostate cell growth
-
-
?
additional information
?
-
-
the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity
-
-
?
additional information
?
-
-
the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity
-
-
?
additional information
?
-
-
no activity with 24,25,26,27-tetranor-23-hydroxyvitamin D3 and vitamin D3
-
-
?
additional information
?
-
-
PTH and PTHrP are involved in enzyme regulation, PTH is the major protein hormone regulating calcium homeostasis, PTHrP is a PTH-like factor responsible for humoral hypercalcemia of malignancy, both peptides are required to achieve normal fetal skeletal morphogenesis, overview
-
-
?
additional information
?
-
-
1alpha,25(OH)2-19-nor-D3 is not converted to 1alpha,25(OH)2-19-nor-D3 by CYP27B1
-
-
?
additional information
?
-
-
25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in vesicles undergo 1alpha-hydroxylation with similar kinetics. CYP27B1 also catalyzes 1alpha-hydroxylation of vesicle-associated 24R,25-dihydroxyvitamin D3 and 20-hydroxyvitamin D3, and 25-hydroxylation of 1alpha-hydroxyvitamin D3 and 1alpha-hydroxyvitamin D2, but with much lower efficiency than for 25(OH)D3, reactions in reconstituted phospholipid vesicles with the substrates associating to the membrane, effect of vesicle phospholipid composition on metabolism of 25(OH)D3 by CYP27B1, overview
-
-
?
additional information
?
-
-
a multifunctional enzyme catalyzing also the reaction of cholestanetriol 26-monooxygenase, EC 1.14.13.15, as well as oxidation of 5beta-cholestane-3alpha,7alpha,12alpha,27-tetraol into the corresponding C27-acid, and the 24- and 25-hydroxylation of cholesterol, the truncated enzyme form is less efficient than the full-length CYP27A in the 27-hydroxylation of C27-sterols, and much less efficient in the 25-hydroxylation of 1alpha-hydroxyvitamin D3
-
-
?
additional information
?
-
-
Vdh, a cytochrome P450 enzyme, is responsible for the biocatalytic conversion of vitamin D3, catalyzing the two-step hydroxylation of VD3, i.e. the conversion of VD3 to 25-hydroxyvitamin D3 (25(OH)VD3) and then of 25(OH)VD3 to 1alpha,25(OH)2VD3, a hormonal form of VD3. Anti-parallel substrate binding modes enable sequential hydroxylation, structure-function analysis, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
25-hydroxyvitamin D2 + NADPH + H+ + O2
1,25-dihydroxyvitamin D2 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + H+ + O2
25-hydroxyvitamin D3 + H2O
-
-
-
?
25-hydroxyvitamin D3 + NADPH + H+ + O2
1,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
cholecalciferol + NADPH + O2
25-hydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
additional information
?
-
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
-
-
?
(24R),25-dihydroxycholecalciferol + NADPH + O2
1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
energy-dependent transhydrogenation is of importance
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
enzyme activity is correlated with phosphate content in serum
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
recombinant in E. coli
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
central role in calcium regulation
-
-
?
25-hydroxycholecalciferol + NADPH + O2
calcitriol + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
1,25(OH)2D3 is an antiproliferative agent that may inhibit proliferation of breast cancer cells
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
1,25-dihydroxyvitamin D3 (1,25-D3) is a key determinant of calcium homeostasis, cell proliferation and differentiation, calcium and vitamin D might have a protective function against colorectal tumor pathogenesis, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
bioactivation
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
extra-renal functions of 1alpha-hydroxylase in vitamin D physiology, regulation, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
regulation of enzyme expression in kidney proximal tubules, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
substrate activation by the rate-limiting enzyme 1alpha-hydroxylase in cells of the immune system under control of immune stimuli, such as interferon-gamma, in pathological situations, such as sarcoidosis, this can lead to systemic excess of 1,25(OH)2D3 and hypercalcemia
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
the enzyme hydroxylates the major circulating form of vitamin D to the active systemic hormone 1,25(OH)2D3, local production of 1,25(OH)2D3 appears to occur also at other sites where 1alpha-hydroxylase is expressed for autocrine/paracrine regulation, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
the enzyme is involved in the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate, mechanism, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
the enzyme is responsible for final hydroxylation of vitamin D3, in response to IFNgamma and CD14/TLR4 activation
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
deficiency of the enzyme, due to mutation of residues Q65 and T409, in substrate binding causes vitamin D-dependent rickets type 1, overview
-
-
?
25-hydroxyvitamin D + NADPH + O2
1alpha,25-dihydroxyvitamin D + NADP+ + H2O
-
-
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D, involved in tumor suppressing activity due to the tumor growth inhibiting effect of 1alpha,25-dihydroxyvitamin D3
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
final step of the activation of vitamin D
-
-
?
25-hydroxyvitamin D3 + NADPH + O2
1alpha,25-dihydroxyvitamin D3 + NADP+ + H2O
-
-
-
-
?
additional information
?
-
-
25(OH)D3 therapy is useful for colorectal cancer chemoprevention but provitamin D is less likely to be useful for colorectal cancer chemotherapy, overview
-
-
?
additional information
?
-
-
both butyrate and 1alpha-25(OH)2D3 stimulate differentiation of Caco-2 cells, while 25(OH)2D3 has no impact on cell differentiation
-
-
?
additional information
?
-
-
colonic and renal vitamin D hydroxylases are regulated independently
-
-
?
additional information
?
-
-
functional analysis of vitamin D metabolism in cancer, locally synthesized 1,25(OH)2D3 may be inefficient in preventing tumor progression, mechanism, overview
-
-
?
additional information
?
-
-
immune regulation of the enzyme in monocytes, cross-talk between the JAK-STAT, the NFkappaB, and the p38 MAPK pathways is necessary, and direct binding of C/EBPbeta to its recognition sites in the promoter of the 1alpha-hydroxylase gene is a prerequisite, overview
-
-
?
additional information
?
-
-
the enzyme is involved in local production of 1alpha,25-dihydroxyvitamin D, i.e. 1alpha,25(OH)2D3, in tumorigenesis
-
-
?
additional information
?
-
-
the 1alpha-OHase gene is a tumor suppressor gene responsible for the normal regulation of prostate cell growth
-
-
?
additional information
?
-
-
PTH and PTHrP are involved in enzyme regulation, PTH is the major protein hormone regulating calcium homeostasis, PTHrP is a PTH-like factor responsible for humoral hypercalcemia of malignancy, both peptides are required to achieve normal fetal skeletal morphogenesis, overview
-
-
?
additional information
?
-
-
Vdh, a cytochrome P450 enzyme, is responsible for the biocatalytic conversion of vitamin D3, catalyzing the two-step hydroxylation of VD3, i.e. the conversion of VD3 to 25-hydroxyvitamin D3 (25(OH)VD3) and then of 25(OH)VD3 to 1alpha,25(OH)2VD3, a hormonal form of VD3. Anti-parallel substrate binding modes enable sequential hydroxylation, structure-function analysis, overview
-
-
?
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Yoon, P.S.; DeLuca, H.F.
Purification and properties of vitamin D hydroxylases
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430-440
1980
Gallus gallus
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25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties
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247
7528-7532
1972
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Botham, K.M.; Tanaka, Y.; DeLuca, H.F.
25-Hydroxyvitamin D3-1-hydroxylase. Inhibition in vitro by rat and pig tissues
Biochemistry
13
4961-4966
1974
Gallus gallus
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Ghazarian, J.G.; Kream, B.; Botham, K.M.; Nickells, M.W.; DeLuca, H.F.
Rat plasma 25-hydroxyvitamin D3 binding protein: an inhibitor of the 25-hydroxyvitamin D3-1alpha-hydroxylase
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189
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1978
Gallus gallus
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Studies on calciferol metabolism. Regulation of 25-hydroxyvitamin D3-1-hydroxylase in isolated renal mitochondria
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172
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1976
Gallus gallus
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Botham, K.M.; Ghazarian, J.G.; Kream, B.E.; DeLuca, H.F.
Isolation of an inhibitor of 25-hydroxyvitamin D3-1-hydroxylase from rat serum
Biochemistry
15
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1976
Gallus gallus
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Paulson, S.K.; DeLuca, H.F.
Subcellular location and properties of rat renal 25-hydroxyvitamin D3-1 alpha-hydroxylase
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260
11488-11492
1985
Rattus norvegicus
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Vieth, R.; Fraser, D.
Kinetic behavior of 25-hydroxyvitamin D-1-hydroxylase and -24-hydroxylase in rat kidney mitochondria
J. Biol. Chem.
254
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1979
Rattus norvegicus
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Ghazarian, J.G.; Yanda, D.M.
Inhibition of 25-hydroxyvitamin D 1 alpha-hydroxylase by renal mitochondrial protein kinase-catalyzed phosphorylation
Biochem. Biophys. Res. Commun.
132
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1985
Gallus gallus
brenda
Ghazarian, J.G.; Jefcoate, C.R.; Knutson, J.C.; Orme-Johnson, W.H.; DeLuca, H.F.
Mitochondrial cytochrome P450. A component of chick kidney 25-hydrocholecalciferol-1alpha-hydroxylase
J. Biol. Chem.
249
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1974
Gallus gallus
brenda
Henry, H.L.; Norman, A.W.
Studies on calciferol metabolism. IX. Renal 25-hydroxy-vitamin D3-1 hydroxylase. Involvement of cytochrome P-450 and other properties
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249
7529-7535
1974
Gallus gallus
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Purification and characterization of the ferredoxin component of 25-hydroxycholecalciferol 1 alpha-hydroxylase
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177
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1979
Gallus gallus
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Gray, R.W.; Ghazarian, J.G.
Solubilization and reconstitution of kidney 25-hydroxyvitamin D3 1 alpha- and 24-hydroxylases from vitamin D-replete pigs
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259
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1989
Sus scrofa
brenda
Pedersen, J.I.; Ghazarian, J.G.; Orme-Johnson, N.R.; DeLuca, H.F.
Isolation of chick renal mitochondrial ferredoxin active in the 25-hydroxyvitamin D3-1alpha-hydroxylase system
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251
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1976
Gallus gallus
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Postlind, H.
Separation of the cytochromes P-450 in pig kidney mitochondria catalyzing 1 alpha-, 24- and 26-hydroxylations of 25-hydroxyvitamin D3
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168
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1990
Sus scrofa
brenda
Hiwatashi, A.; Nishii, Y.; Ichikawa, Y.
Purification of cytochrome P-450D1 alpha (25-hydroxyvitamin D3-1 alpha-hydroxylase) of bovine kidney mitochondria
Biochem. Biophys. Res. Commun.
105
320-327
1982
Bos taurus
brenda
Hagenfeldt, Y.; Pedersen, J.I.; Bjrkhem, I.
Properties of guinea-pig kidney 25-hydroxyvitamin D3 1 alpha-hydroxylase assayed by isotope dilution-mass spectrometry
Biochem. J.
250
521-526
1988
Cavia porcellus
brenda
Paulson, S.K.; Phelps, M.; DeLuca, H.F.
Assay and properties of rat yolk sac 25-hydroxyvitamin D3 1 alpha-hydroxylase
Biochemistry
25
6821-6826
1986
Rattus norvegicus
brenda
Lobaugh, B.; Almond, J.R.; Drezner, M.K.
Measurement of 25-hydroxyvitamin D 1-alpha-hydroxylase activity in mammalian kidney
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123
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1986
Mammalia
brenda
Delvin, E.E.; Arabian, A.
Kinetics and regulation of 25-hydroxycholecalciferol 1 alpha-hydroxylase from cells isolated from human term decidua
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163
659-662
1987
Homo sapiens
brenda
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Immunopurified 25-hydroxyvitamin D 1a-hydroxylase and 1,25-dihydroxyvitamin D 24-hydroxylase are closely related but distinct enzymes
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267
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1992
Gallus gallus
brenda
Grieff, M.; Dusso, A.; Mori, T.; Nishii, Y.; Slatopolsky, E.; Brown, A.J.
22-Oxacalcitriol suppresses 25-hydroxycholecalciferol-1a-hydroxylase in rat kidney
Biochem. Biophys. Res. Commun.
185
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1992
Rattus norvegicus
brenda
Daniel, D.; Middleton, R.; Henry, H.L.; Okamura, W.H.
Inhibitors of 25-hydroxyvitamin D3 1a-hydroxylase: A-ring oxa analogs of 25-hydroxyvitamin D3
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61
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1996
Gallus gallus
-
brenda
Nakamura, Y.; Eto, T.A.; Taniguchi, T.; Miyamoto, K.; Nagatomo, J.; Shiotsuki, H.; Sueta, H.; Higashi, S.; Okuda, K.I.; Setoguchi, T.
Purification and characterization of 25-hydroxyvitamin D3 1alpha-hydroxylase from rat kidney mitochondria
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419
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1997
Rattus norvegicus
brenda
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25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis
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277
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1997
Mus musculus (O35084)
brenda
Sawada, N.; Sakaki, T.; Kitanaka, S.; Takeyama, K.i.; Kato, S.; Inouye, K.
Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase: coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli
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265
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1999
Homo sapiens
brenda
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Enzymic properties of mouse 25-hydroxyvitamin D3 1alpha-hydroxylase expressed in Escherichia coli
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259
731-738
1999
Mus musculus
brenda
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Cloning of porcine 25-hydroxyvitamin D3 1alpha-hydroxylase and its regulation by cAMP in LLC-PK1 cells
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10
963-970
1999
Sus scrofa (Q9XS57), Sus scrofa
brenda
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The human renal 25-hydroxyvitamin D3-1a-hydroxylase: properties studied by isotope-dilution mass spectrometry
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22
223-228
1992
Homo sapiens
brenda
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Clonal differences in expression of 25-hydroxyvitamin D(3)-1alpha-hydroxylase, of 25-hydroxyvitamin D(3)-24-hydroxylase, and of the vitamin D receptor in human colon carcinoma cells: effects of epidermal growth factor and 1alpha,25-dihydroxyvitamin D(3)
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276
320-327
2002
Homo sapiens
brenda
Inouye, K.; Sakaki, T.
Enzymatic studies on the key enzymes of vitamin D metabolism; 1alpha-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24)
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7
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2001
Homo sapiens, Mus musculus
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Expression and activity of 25-hydroxyvitamin D-1alpha-hydroxylase are restricted in cultures of human syncytiotrophoblast cells from preeclamptic pregnancies
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87
3876-3882
2002
Homo sapiens
brenda
Hosseinpour, F.; Norlin, M.; Wikvall, K.
Kidney microsomal 25- and 1alpha-hydroxylase in vitamin D metabolism: catalytic properties, molecular cloning, cellular localization and expression during development
Biochim. Biophys. Acta
1580
133-144
2002
Sus scrofa
brenda
Somjen, D.; Weisman, Y.; Kohen, F.; Gayer, B.; Limor, R.; Sharon, O.; Jaccard, N.; Knoll, E.; Stern, N.
25-Hydroxyvitamin D3-1alpha-hydroxylase is expressed in human vascular smooth muscle cells and is upregulated by parathyroid hormone and estrogenic compounds
Circulation
111
1666-1671
2005
Homo sapiens
brenda
Panda, D.K.; Miao, D.; Bolivar, I.; Li, J.; Huo, R.; Hendy, G.N.; Goltzman, D.
Inactivation of the 25-hydroxyvitamin D 1alpha-hydroxylase and vitamin D receptor demonstrates independent and interdependent effects of calcium and vitamin D on skeletal and mineral homeostasis
J. Biol. Chem.
279
16754-16766
2004
Mus musculus
brenda
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Conventional and tissue-specific inactivation of the 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1)
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88
245-251
2003
Mus musculus
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Eyles, D.W.; Smith, S.; Kinobe, R.; Hewison, M.; McGrath, J.J.
Distribution of the vitamin D receptor and 1alpha-hydroxylase in human brain
J. Chem. Neuroanat.
29
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2005
Homo sapiens
brenda
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Towards a complete picture of splice variants of the gene for 25-hydroxyvitamin D3 1alpha-hydroxylase in brain and skin cancer
J. Steroid Biochem. Mol. Biol.
89-90
527-532
2004
Homo sapiens
brenda
Khorchide, M.; Lechner, D.; Cross, H.S.
Epigenetic regulation of vitamin D hydroxylase expression and activity in normal and malignant human prostate cells
J. Steroid Biochem. Mol. Biol.
93
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2005
Homo sapiens
brenda
Ma, J.F.; Nonn, L.; Campbell, M.J.; Hewison, M.; Feldman, D.; Peehl, D.M.
Mechanisms of decreased Vitamin D 1alpha-hydroxylase activity in prostate cancer cells
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221
67-74
2004
Homo sapiens
brenda
Friedrich, M.; Diesing, D.; Cordes, T.; Fischer, D.; Becker, S.; Chen, T.C.; Flanagan, J.N.; Tangpricha, V.; Gherson, I.; Holick, M.F.; Reichrath, J.
Analysis of 25-hydroxyvitamin D3-1alpha-hydroxylase in normal and malignant breast tissue
Anticancer Res.
26
2615-2620
2006
Homo sapiens
brenda
Furster, C.; Bergman, T.; Wikvall, K.
Biochemical characterization of a truncated form of CYP27A purified from rabbit liver mitochondria
Biochem. Biophys. Res. Commun.
263
663-666
1999
Oryctolagus cuniculus
brenda
Segersten, U.; Holm, P.K.; Bjrklund, P.; Hessman, O.; Nordgren, H.; Binderup, L.; Akerstrm, G.; Hellman, P.; Westin, G.
25-Hydroxyvitamin D3 1alpha-hydroxylase expression in breast cancer and use of non-1alpha-hydroxylated vitamin D analogue
Breast Cancer Res.
7
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2005
Homo sapiens (O15528)
brenda
Matusiak, D.; Murillo, G.; Carroll, R.E.; Mehta, R.G.; Benya, R.V.
Expression of vitamin D receptor and 25-hydroxyvitamin D3-1{alpha}-hydroxylase in normal and malignant human colon
Cancer Epidemiol. Biomarkers Prev.
14
2370-2376
2005
Homo sapiens
brenda
Kallay, E.; Bises, G.; Bajna, E.; Bieglmayer, C.; Gerdenitsch, W.; Steffan, I.; Kato, S.; Armbrecht, H.J.; Cross, H.S.
Colon-specific regulation of vitamin D hydroxylases - a possible approach for tumor prevention
Carcinogenesis
26
1581-1589
2005
Homo sapiens
brenda
Yamamoto, K.; Uchida, E.; Urushino, N.; Sakaki, T.; Kagawa, N.; Sawada, N.; Kamakura, M.; Kato, S.; Inouye, K.; Yamada, S.
Identification of the amino acid residue of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D-dependent rickets type 1
J. Biol. Chem.
280
30511-30516
2005
Mus musculus
brenda
Xue, Y.; Zhang, Z.; Karaplis, A.C.; Hendy, G.N.; Goltzman, D.; Miao, D.
Exogenous PTH-related protein and PTH improve mineral and skeletal status in 25-hydroxyvitamin D-1alpha-hydroxylase and PTH double knockout mice
J. Bone Miner. Res.
20
1766-1777
2005
Mus musculus
brenda
Stoffels, K.; Overbergh, L.; Giulietti, A.; Verlinden, L.; Bouillon, R.; Mathieu, C.
Immune regulation of 25-hydroxyvitamin-D3-1alpha-hydroxylase in human monocytes
J. Bone Miner. Res.
21
37-47
2006
Homo sapiens
brenda
Overbergh, L.; Stoffels, K.; Waer, M.; Verstuyf, A.; Bouillon, R.; Mathieu, C.
Immune regulation of 25-hydroxyvitamin D-1alpha-hydroxylase in human monocytic THP1 cells: mechanisms of interferon-gamma-mediated induction
J. Clin. Endocrinol. Metab.
91
3566-3574
2006
Homo sapiens (O15528), Homo sapiens
brenda
Khadzkou, K.; Buchwald, P.; Westin, G.; Dralle, H.; Akerstroem, G.; Hellman, P.
25-hydroxyvitamin D3 1alpha-hydroxylase and vitamin D receptor expression in papillary thyroid carcinoma
J. Histochem. Cytochem.
54
355-361
2006
Homo sapiens
brenda
Hendrix, I.; Anderson, P.H.; Omdahl, J.L.; May, B.K.; Morris, H.A.
Response of the 5-flanking region of the human 25-hydroxyvitamin D 1alpha-hydroxylase gene to physiological stimuli using a transgenic mouse model
J. Mol. Endocrinol.
34
237-245
2005
Homo sapiens
brenda
Carneiro de Lyra, E.; Amorim da Silva, I.; Katayama, M.L.; Brentani, M.M.; Nonogaki, S.; Goes, J.C.; Folgueira, M.A.
25(OH)D3 and 1,25(OH)2D3 serum concentration and breast tissue expression of 1alpha-hydroxylase, 24-hydroxylase and Vitamin D receptor in women with and without breast cancer
J. Steroid Biochem. Mol. Biol.
100
184-192
2006
Homo sapiens
brenda
Townsend, K.; Evans, K.N.; Campbell, M.J.; Colston, K.W.; Adams, J.S.; Hewison, M.
Biological actions of extra-renal 25-hydroxyvitamin D-1alpha-hydroxylase and implications for chemoprevention and treatment
J. Steroid Biochem. Mol. Biol.
97
103-109
2005
Homo sapiens
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Schroeder, O.; Turak, S.; Daniel, C.; Gaschott, T.; Stein, J.
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