required, the conserved coenzyme binding motif (GxGxxG) and zinc-ADH signature (GHExxGxxxxxGxxV) are observed in the amino acid sequence of RpXDH at position 181-186 and 70-84. The ligand binding residues for catalytic zinc (residue C46, H71, E72, and E157) and structural zinc (residue C101, C104, C107, and C115) are found in the RpXDH sequence
the conserved coenzyme binding motif (GxGxxG) and zinc-ADH signature (GHExxGxxxxxGxxV) are observed in the amino acid sequence of RpXDH at position 181-186 and 70-84 and are completely conserved among RpXDH, XDHs, and SDHs from other filamentous fungi and yeasts
the conserved coenzyme binding motif (GxGxxG) and zinc-ADH signature (GHExxGxxxxxGxxV) are observed in the amino acid sequence of RpXDH at position 181-186 and 70-84 and are completely conserved among RpXDH, XDHs, and SDHs from other filamentous fungi and yeasts
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from Rhizomucor pusillus strain NBRC 4578 by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration, and a another different step of anion exchange chromatography, ultrafiltration, and gel filtration. The next purification steps are Reactive Red 120 affinity chromatography, dialysis, and ultrafiltration. Recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
the enzyme might be useful for production biofuels from D-xylose. Chlorella sorokiniana can uptake D-xylose only by an inducible D-xylose transportation system in a light-dependent manner after induction with D-glucose. Xylose reductase (XDH) then converts 50 to 60% of the consumed D-xylose to xylitol, which is subsequently converted to D-xylulose
the enzyme might be useful for production biofuels from D-xylose. Chlorella sorokiniana can uptake D-xylose only by an inducible D-xylose transportation system in a light-dependent manner after induction with D-glucose. Xylose reductase (XDH) then converts 50 to 60% of the consumed D-xylose to xylitol, which is subsequently converted to D-xylulose
Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP+-linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana