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4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
cinnamyl acetate + NADPH + H+
? + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
additional information
?
-
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
GC-MS analysis product analysis
-
?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
r
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
r
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
?
4-coumaryl acetate + NADPH + H+
chavicol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
CbEGS1 is specific for isoeugenol production
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
GC-MS analysis product analysis
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
GC-MS analysis product analysis
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
ObEGS1 uses a quinone methide intermediate-based mechanism to generate an intermediate to which the reductive transfer of the reducing hydride can then be easily accomplished. the enzyme acts on the substrate via a push-pull mechanism, removing the proton of the para hydroxyl group and promoting the cleavage of the acetyl group. In the resultant quinone-methide intermediate, the C7 atom serves as the acceptor of the hydride ion from NADPH
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
reaction via a quinone methide-like intermediate
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
PhEGS1 converts coniferyl acetate to eugenol
-
-
?
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
r
coniferyl acetate + NADPH + H+
eugenol + acetate + NADP+
-
-
-
-
r
additional information
?
-
purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate
-
-
-
additional information
?
-
Gymnadenia odoratissima proteins synthesize eugenol only
-
-
?
additional information
?
-
Gymnadenia odoratissima proteins synthesize eugenol only
-
-
?
additional information
?
-
-
Gymnadenia odoratissima proteins synthesize eugenol only
-
-
?
additional information
?
-
Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
-
-
?
additional information
?
-
Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
-
-
?
additional information
?
-
-
Gymnadenia densiflora proteins synthesize eugenol, as well as a smaller amount of isoeugenol
-
-
?
additional information
?
-
specificity of EGS for the production of allyl phenols in heterologically EGS expressing Fragaria x ananassa fruits
-
-
?
additional information
?
-
determinants of the regioselectivity of the EGS-catalyzed reduction reaction, overview
-
-
?
additional information
?
-
-
determinants of the regioselectivity of the EGS-catalyzed reduction reaction, overview
-
-
?
additional information
?
-
no substrate: 4-coumaryl acetate, cinnamyl acetate
-
-
?
additional information
?
-
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes
-
-
-
additional information
?
-
no substrate: cinnamyl acetate
-
-
?
additional information
?
-
no substrate: cinnamyl acetate
-
-
?
additional information
?
-
no substrate: cinnamyl acetate
-
-
?
additional information
?
-
-
no substrate: cinnamyl acetate
-
-
?
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brenda
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brenda
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-
brenda
-
brenda
-
brenda
-
the highest specific activity levels of eugenol synthase are found in the stamens, followed closely by the pistil and petals
brenda
expression in floral tissue
brenda
expression in floral tissue
brenda
-
-
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
-
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
-
-
brenda
-
PhEGS1 is expressed specifically in the scent-producing parts of the flowers, limbs and tube, but not in other parts of the flowers nor in leaves
brenda
mainly detected in stamens and petals at the flower opening stage. RcEGS1 is principally localized in the upper and lower epidermal layers, which are the major sites of scent emission in roses
brenda
-
mainly detected in stamens and petals at the flower opening stage
brenda
young and mature leaves
brenda
-
-
brenda
-
brenda
-
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
in leaves, not in leaf stripped of PGTs
brenda
leaves and leaves stripped of PGTs
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
young and mature, gene egs1 is expressed in young leaves
brenda
higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents
brenda
PGT
brenda
PGTs
brenda
-
-
brenda
enzyme RcEGS1 has its highest expression levels in the petals compared to other tissues. It has higher transcript levels at the developmental blooming stage and lower levels at budding and senescence stages
brenda
-
-
brenda
-
brenda
the amount of eugenol produced in roots of aseptically-grown plants under elicitation is much lower than the amount produced in roots of soil-grown plants. This might be because of the different external factors and multiple stress conditions experienced in soil as compared to aseptic conditions
brenda
-
-
brenda
-
brenda
-
-
brenda
additional information
quantitative RT-PCR enzyme expression analysis in different tissues. Distribution of aromatic volatiles in different parts of the carrot plants, overview
brenda
additional information
no expression in leaf tissue
brenda
additional information
no expression in leaf tissue
brenda
additional information
-
no expression in leaf tissue
brenda
additional information
no expression in leaf tissue
brenda
additional information
no expression in leaf tissue
brenda
additional information
-
no expression in leaf tissue
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes
brenda
additional information
-
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs
brenda
additional information
no expression of EGS1 in roots
brenda
additional information
-
no expression of EGS1 in roots
brenda
additional information
unlike camphor, eugenol accumulates more in roots. But the absence of the eugenol synthase gene in roots indicates long distance transport from aerial tissues. In silico co-expression analysis indicates the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Ocimum kilimandscharicum roots accumulate a large amount of eugenol (56.6%) with low amount of camphor (16%). Gene egs1 is expressed in young leaves and flowers
brenda
additional information
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unlike camphor, eugenol accumulates more in roots. But the absence of the eugenol synthase gene in roots indicates long distance transport from aerial tissues. In silico co-expression analysis indicates the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Ocimum kilimandscharicum roots accumulate a large amount of eugenol (56.6%) with low amount of camphor (16%). Gene egs1 is expressed in young leaves and flowers
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malfunction
downregulation of RcEGS1 expression in flowers by virus-induced gene silencing leads to a reduction of the relative content of eugenol compared to wild-type flowers
evolution
EGS is structurally related to the shortchain dehydrogenase/reductases, SDRs, and in particular, enzymes in the isoflavone-reductase-like subfamily
evolution
the phenylpropene-forming eugenol synthase, EGS, belongs to a structural family of NADPH-dependent reductases that also includes isoeugenol synthase, IGS, pinoresinol-lariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview
evolution
Ocimum basilicum contains 8 isozymes of eugenol synthase, EGS. The enzymes belong to the PIP family, named after the first three identified members, pinoresinollariciresinol reductase (PLR), isoflavone reductase (IFR), and phenylcoumaran benzylic ether reductase (PCBER)
metabolism
the enzyme is involved in the biosynthesis of phenylpropenes, overview
metabolism
PGT-enriched BAHD acyltransferases, encoded by genes ObCAAT1 and ObCAAT2, convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis, followed by reduction of coniferyl acetate to eugenol and acetate catalyzed by eugenol synthase EGS1. Both ObCAAT1 and ObCAAT2 in combination with ObEGS1 produce eugenol The phenylpropene biosynthesis is not coupled to lignification in sweet basil. Plant phenylpropanoid pathway, overview
metabolism
unlike camphor, eugenol accumulates more in roots. But the absence of the eugenol synthase gene in roots indicates long distance transport from aerial tissues. In silico co-expression analysis indicates the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Ocimum spp. display metabolite partitioning between aerial and root tissues, strict partitioning of metabolites between the aerial shoot system (including young leaves, mature leaves, inflorescence, and flowers) and underground root system. Tissue-specific expression of terpenoid and phenylpropanoid pathway related genes in Ocimum kilimandscharicum. Transcriptomic analysis revealed the highest egs1 expression in young leaves followed by that in flowers, both of which accumulated considerably less amounts of eugenol. The sugar transporter(s) might be involved in partitioning eugenol in roots
physiological function
catalytic involvement in EGS of the conserved Lys132 in preparing the phenolic substrate for quinone methide formation through the proton-relay network
physiological function
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plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
physiological function
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plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
physiological function
introduction of petunia coniferyl alcohol acetyltransferase (CFAT) and eugenol synthase (EGS) into hybrid aspen. The overexpression of EGS alone results in a subtle increase in either eugenol or eugenol glycosides, and the overexpression of both CFAT and EGS results in significant increases in the levels of both eugenol and eugenol glycosides which are nonetheless lower than the increases seen with overexpression of CFAT alone
physiological function
enzyme is responsible for eugenol biosynthesis in roses
physiological function
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enzyme is responsible for eugenol biosynthesis in roses. Major volatile compounds from flower of Rosa x hybrida cv. Yunxiang at stage 4 are analyzed by SPME-GC/MS, the highest amount of 2-phenylethanol, followed by eugenol and methyleugenol, overview
physiological function
eugenol/isoeugenol synthase (DcE(I)GS1) is an NADPH-dependent enzyme that converts coniferyl acetate to eugenol. This enzyme exhibits dual product specificity and yields propenylphenol isoeugenol alongside allylphenol eugenol
physiological function
sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase
additional information
structure of a ternary complex of EGS bound to the cofactor NADP(H) and a mixed competitive inhibitor (7S,8S)-ethyl (7,8-methylene)-dihydroferulate, binding interactions within the EGS active site, overview
additional information
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structure of a ternary complex of EGS bound to the cofactor NADP(H) and a mixed competitive inhibitor (7S,8S)-ethyl (7,8-methylene)-dihydroferulate, binding interactions within the EGS active site, overview
additional information
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the enzyme is a PIP reductase homologue
additional information
distribution of aromatic volatiles in different parts of the carrot plants, overview
additional information
ObCAAT1-RNAi transgenic lines show decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives
additional information
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ObCAAT1-RNAi transgenic lines show decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives
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DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in GST-tagged Escherichia coli strain BL21
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DNA and amino acid sequence determination and analysis, sequence comparison, quantitative expression analysis
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DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
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DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of nontagged and of His-tagged CbEGS1 and CbEGS2 in Escherichia coli
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DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative expression analysis
EGS1 expression in Fragaria x ananassa cv. Elsanta via transfection with Agrobacterium tumefaciens strain AGL0, downregulation of chalcone synthase via antisense construct in parallel
gene DcEGS1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli
gene EGS1, cloning from petals of Rosa chinensis cv. Old Blush, DNA and amino acid sequence determination, recombinant overexpression of His-tagged enzyme in Escherichia coli from pTRV2 vector
gene EGS1, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4
gene egs1, quantitative real-time PCR enzyme expression analysis
gene EGS1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3). Transient coexpression of ObCAAT1 and/o ObCAAT2 with ObEGS1 under control of CMV 35S promoter in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection, the transgenic leaves produce eugenol only when external coniferyl alcohol is added
gene EGS2, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS3, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS4, genotyping and quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4
gene EGS5, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS6, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS7, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
gene EGS8, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter
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Vassao, D.; Kim, S.; Milhollan, J.; Eichinger, D.; Davin, L.; Lewis, N.
A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentata) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenols chavicol/eugenol, but not the propenylphenols p-anol/isoeug
Arch. Biochem. Biophys.
465
209-218
2007
Larrea tridentata
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Hoffmann, T.; Kurtzer, R.; Skowranek, K.; Kiessling, P.; Fridman, E.; Pichersky, E.; Schwab, W.
Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway
Metab. Eng.
13
527-531
2011
Ocimum basilicum (Q15GI4)
brenda
Koeduka, T.; Louie, G.V.; Orlova, I.; Kish, C.M.; Ibdah, M.; Wilkerson, C.G.; Bowman, M.E.; Baiga, T.J.; Noel, J.P.; Dudareva, N.; Pichersky, E.
The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages
Plant J.
54
362-374
2008
Clarkia breweri, Ocimum basilicum, Petunia x hybrida
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Koeduka, T.; Orlova, I.; Baiga, T.J.; Noel, J.P.; Dudareva, N.; Pichersky, E.
The lack of floral synthesis and emission of isoeugenol in Petunia axillaris subsp. parodii is due to a mutation in the isoeugenol synthase gene
Plant J.
58
961-969
2009
Petunia axillaris subsp. parodii
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Louie, G.V.; Baiga, T.J.; Bowman, M.E.; Koeduka, T.; Taylor, J.H.; Spassova, S.M.; Pichersky, E.; Noel, J.P.
Structure and reaction mechanism of basil eugenol synthase
PLoS ONE
2
e993
2007
Ocimum basilicum (Q15GI4), Ocimum basilicum
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Koeduka, T.; Fridman, E.; Gang, D.; Vassao, D.; Jackson, B.; Kish, C.; Orlova, I.; Spassova, S.; Lewis, N.; Noel, J.; Baiga, T.; Dudareva, N.; Pichersky, E.
Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester
Proc. Natl. Acad. Sci. USA
103
10128-10133
2006
Ocimum basilicum (Q15GI4)
brenda
Koeduka, T.; Suzuki, S.; Iijima, Y.; Ohnishi, T.; Suzuki, H.; Watanabe, B.; Shibata, D.; Umezawa, T.; Pichersky, E.; Hiratake, J.
Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering
Biochem. Biophys. Res. Commun.
436
73-78
2013
Petunia x hybrida (B2WSN1)
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Anand, A.; Jayaramaiah, R.H.; Beedkar, S.D.; Singh, P.A.; Joshi, R.S.; Mulani, F.A.; Dholakia, B.B.; Punekar, S.A.; Gade, W.N.; Thulasiram, H.V.; Giri, A.P.
Comparative functional characterization of eugenol synthase from four different Ocimum species: Implications on eugenol accumulation
Biochim. Biophys. Acta
1864
1539-1547
2016
Ocimum basilicum (A0A1B2U6R8), Ocimum tenuiflorum (A0A1B2U6S6), Ocimum tenuiflorum, Ocimum gratissimum (A0A1B2U6S7), Ocimum kilimandscharicum (A0A1B2U6T4)
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Gupta, A.K.; Schauvinhold, I.; Pichersky, E.; Schiestl, F.P.
Eugenol synthase genes in floral scent variation in Gymnadenia species
Funct. Integr. Genomics
14
779-788
2014
Gymnadenia x densiflora (A0A0E3H852), Gymnadenia x densiflora (A0A0E3KNP5), Gymnadenia x densiflora, Gymnadenia odoratissima (A0A0E3NBN0), Gymnadenia odoratissima (A0A0E3NCN9), Gymnadenia odoratissima
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Medina-Puche, L.; Molina-Hidalgo, F.J.; Boersma, M.; Schuurink, R.C.; Lopez-Vidriero, I.; Solano, R.; Franco-Zorrilla, J.M.; Caballero, J.L.; Blanco-Portales, R.; Munoz-Blanco, J.
An R2R3-MYB transcription factor regulates eugenol production in ripe strawberry fruit receptacles
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168
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2015
Fragaria x ananassa (T2AZ69), Fragaria x ananassa
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Reddy, V.A.; Li, C.; Nadimuthu, K.; Tjhang, J.G.; Jang, I.C.; Rajani, S.
Sweet basil has distinct synthases for eugenol biosynthesis in glandular trichomes and roots with different regulatory mechanisms
Int. J. Mol. Sci.
22
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2021
Ocimum basilicum, Ocimum basilicum (A0A1B2U6R8), Ocimum basilicum (A0A1B2U6S4), Ocimum basilicum (A0A1B2U6T3), Ocimum basilicum (A0A7S9C1H0), Ocimum basilicum (A0A7S9C1M1), Ocimum basilicum (A0A7U3PJH6), Ocimum basilicum (Q15GI4)
brenda
Dhar, N.; Sarangapani, S.; Reddy, V.A.; Kumar, N.; Panicker, D.; Jin, J.; Chua, N.H.; Sarojam, R.
Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis
J. Exp. Bot.
71
3638-3652
2020
Ocimum basilicum (Q15GI4), Ocimum basilicum
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Yahyaa, M.; Berim, A.; Nawade, B.; Ibdah, M.; Dudareva, N.; Ibdah, M.
Biosynthesis of methyleugenol and methylisoeugenol in Daucus carota leaves characterization of eugenol/isoeugenol synthase and O-methyltransferase
Phytochemistry
159
179-189
2019
Daucus carota subsp. sativus (A0A166G7A0)
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Singh, P.; Kalunke, R.M.; Shukla, A.; Tzfadia, O.; Thulasiram, H.V.; Giri, A.P.
Biosynthesis and tissue-specific partitioning of camphor and eugenol in Ocimum kilimandscharicum
Phytochemistry
177
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2020
Ocimum kilimandscharicum (A0A1B2U6T4), Ocimum kilimandscharicum
brenda
Yan, H.; Baudino, S.; Caissard, J.C.; Florence Nicole, J.C.; Zhang, H.; Tang, K.; Li, S.; Lu, S.
Functional characterization of the eugenol synthase gene (RcEGS1) in rose
Plant Physiol. Biochem.
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2018
Rosa hybrid cultivar, Rosa chinensis (M1FGX5)
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Rezaie, R.; Abdollahi Mandoulakani, B.; Fattahi, M.
Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L
Sci. Rep.
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2020
Ocimum basilicum (Q15GI4), Ocimum basilicum
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