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Information on EC 1.1.1.243 - carveol dehydrogenase
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1.1.1.243 carveol dehydrogenaseSpecify your search results
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
carveol dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Cdh
dehydrogenase, (-)-trans-carveol
-
-
-
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Cdh
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+
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-
-
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(-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+
steroselective conversion of (6S)-carveol stereomers from a diastereomeric mixture of (4R)- or (4S)-carveol
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(-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+
steroselective conversion of (6S)-carveol stereomers from a diastereomeric mixture of (4R)- or (4S)-carveol
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
redox reaction
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-
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reduction
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oxidation
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PATHWAY SOURCE
PATHWAYS
KEGG
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-
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SYSTEMATIC NAME
IUBMB Comments
(-)-trans-carveol:NADP+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
122653-66-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1R,2R,4S)-limonene-1,2-diol + NAD+
?
-
15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(1S,2S,4R)-limonene-1,2-diol + NAD+
?
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25% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(4R,6S)-dihydrocarveol + NAD+
(4R)-carvone + NADH
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20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(4S,6S)-dihydrocarveol + NAD+
(4S)-carvone + NADH
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20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(R)-indanol + NAD+
indanone + NADH
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13% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(S)-indanol + NAD+
indanon + NADH
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30% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
2-ethylcyclohexanol + NAD+
2-ethylcyclohexanone + NADH
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110% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
2-methylcyclohexanol + NAD+
2-methylcyclohexanone + NADH
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100% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
3,3,5,5-tetramethylcyclohexanol + NAD+
3,3,5,5-tetramethylcyclohexanone + NADH
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55% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
3,5,5-trimethylcyclohex-2-en-1-ol + NAD+
3,5,5-trimethylcyclohex-2-en-1-one + NADH
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115% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
4-ethylcyclohexanol + NAD+
4-ethylcyclohexanone + NADH
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40% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
4-methylcyclohexanol + NAD+
4-methylcyclohexanone + NADH
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35% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
cyclohex-2-ene-1-ol + NAD+
cyclohex-2-ene-1-one + NADH
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10% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
sobrerol + NAD+
?
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20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(-)-trans carveol + NAD+
(-)-carvone + NADH
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stereospecific for the trans-form
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?
(-)-trans carveol + NAD+
(-)-carvone + NADH
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stereospecific for the trans-form
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-
?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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-
?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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-
?
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known
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ir
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known
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ir
(4R,6S)-trans-carveol + NADP+
(4R)-carvone + NADPH
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monoterpene synthesis in mints
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?
(4S,6RS)-carveol + NAD+
(4S)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
(4S,6RS)-carveol + NAD+
(4S)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
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165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
3-methylcyclohexanol + NAD+
3-methylcyclohexanone + NADH
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165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
cyclohexanol + NAD+
cyclohexanone + NADH
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15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
cyclohexanol + NAD+
cyclohexanone + NADH
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15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor
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ir
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4R,6S)-trans-carveol + NADP+
(4R)-carvone + NADPH
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monoterpene synthesis in mints
-
?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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-
?
(-)-trans carveol + NADP+
(-)-carvone + NADPH
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last step of (-)-carvone biosynthesis
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?
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known
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ir
(4R,6S)-trans-carveol + NAD+
(4R)-carvone + NADH
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enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known
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ir
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
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each monomer of carveol dehydrogenase contains one tightly bound NAD(H) molecule
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Cyclohexanol
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induction during growth, slightly more effective than (+)-limonene
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.002
0.005
0.009
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NAD+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.014
0.017
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NAD+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.021
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NAD+-dependent(4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
0.026
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activity in whole cells grown on limonene, biphasic assay system consisting of 20 ml phosphate buffer and 4 ml iso-octane
0.045
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NAD+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
0.09
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dichlorophenolindophenol-dependent(4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.105
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dichlorophenolindophenol+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.002
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NADP+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.002
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NADP+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
0.005
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NADP+-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-limonene
0.005
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NADP+-dependent (4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
0.014
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dichlorphenolindophenol+-dependent(4R)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
0.014
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dichlorophenolindophenol-dependent (4S)-carveol dehydrogenase, activity in extracts of cells grown on (4R)-carveol
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 50
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gradual increase in specific activity from 0.55 micromol/min mg to 2.85 micromol/min mg
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme is induced after growth on limonene or carveol
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
-
growth on (+)-limonene or cyclohexanol induces the enzyme
brenda
additional information
-
growth on (+)-limonene or cyclohexanol induces the enzyme
-
brenda
Show AA Sequence and Transmembrane Helices (104 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 1.95 A resolution. STD-NMR demonstrates binding of the potential substrate carveol, the potential product carvone, the inhibitor tricyclazol, and external redox partner 2,6-dichloroindophenol. The enzyme appears to contain a non-exchangeable NAD cofactor and may rely on an external redox partner, rather than cofactor exchange, for multiple turnover
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the installation of the (-)-carvone biosynthesis from dimethyl allyl diphosphate of Menta spicata in Escherichia coli is not successful due to secretion of the intermediate (-)-limonene, after feeding of (-)limonen to recombinant bacteria, (-)-carvone is produced
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nutrition
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evaluation of recombinant industrial scale production of (-)-carvone, the spearmint monoterpene ketone, in Escherichia coli for commercial use, overview
synthesis
additional information
synthesis
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evaluation of large scale production of (-)-carvone by cells of Rhodococcus erythropolis strain DCL14, overview
synthesis
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evaluation of large scale production of (-)-carvone by cells of Rhodococcus erythropolis strain DCL14, overview
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additional information
-
supply of a mixture of (-)-trans- and (-)-cis-carveol to the organism delivers pure (-)-carvone and pure (-)-cis-carveol
additional information
-
supply of a mixture of (-)-trans- and (-)-cis-carveol to the organism delivers pure (-)-carvone and pure (-)-cis-carveol
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gershenzon, J.; Maffei, M.; Croteau, R.
Biochemical and histochemical localization of monoterpene biosynthesis in the glandular trichomes of spearmint (Mentha spicata)
Plant Physiol.
89
1351-1357
1989
Mentha spicata
brenda
van der Werft, M.J.; van der Ven, C.; Barbirato, F.; Eppink, H.M.; de Bont, J.A.M.; van Berkel, W.J.H.
Stereoselective carveol dehydrogenase from Rhodococcus erythropolis DCL14
J. Biol. Chem.
274
26296-26304
1999
Rhodococcus erythropolis, Rhodococcus erythropolis DCL14
brenda
van der Werf, M.J.; Boot, A.M.
Metabolism of carveol and dihydrocarveol in Rhodococcus erythropolis DCL14
Microbiology
146
1129-1141
2000
Rhodococcus erythropolis
brenda
Tecelao, C.S.R.; van Keulen, F.; da Fonseca, M.R.
Development of a reaction system for the selective conversion of (-)-trans-carveol to (-)-carvone with whole cells of Rhodococcus erythropolis DCL14
J. Mol. Catal. , B Enzym.
11
719-724
2001
Rhodococcus erythropolis
-
brenda
Tecelao, C.S.R.; van Keulen, F.; da Fonseca, M.M.R.
Development of a reaction system for the selective conversion of (-)-trans-carveol to (-)-carvone with whole cells of Rhodococcus erythropolis DCL14
J. Mol. Catal. B
11
719-724
2001
Rhodococcus erythropolis, Rhodococcus erythropolis DCL14
-
brenda
Carter, O.A.; Peters, R.J.; Croteau, R.
Monoterpene biosynthesis pathway construction in Escherichia coli
Phytochemistry
64
425-433
2003
Mentha spicata
brenda
Ringer, K.L.; Davis, E.M.; Croteau, R.
Monoterpene metabolism. Cloning, expression, and characterization of (-)-isopiperitenol/(-)-carveol dehydrogenase of peppermint and spearmint
Plant Physiol.
137
863-872
2005
Mentha spicata
brenda
Haft, D.H.; Pierce, P.G.; Mayclin, S.J.; Sullivan, A.; Gardberg, A.S.; Abendroth, J.; Begley, D.W.; Phan, I.Q.; Staker, B.L.; Myler, P.J.; Marathias, V.M.; Lorimer, D.D.; Edwards, T.E.
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Sci. Rep.
7
41074
2017
Mycolicibacterium thermoresistibile (E1C9L4)
brenda
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Release 2023.1 (January 2023)
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