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IUBMB CommentsAlso acts on benzyl (2S,3S)-2-methyl-3-hydroxybutanoate; otherwise highly specific.
The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae
Synonyms
benzyl 2-methyl-3-hydroxybutyrate dehydrogenase,
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benzyl 2-methyl-3-hydroxybutyrate dehydrogenase
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benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + NADP+ = benzyl 2-methyl-3-oxobutanoate + NADPH + H+
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benzyl-(2R,3S)-2-methyl-3-hydroxybutanoate:NADP+ 3-oxidoreductase
Also acts on benzyl (2S,3S)-2-methyl-3-hydroxybutanoate; otherwise highly specific.
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2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
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ratio syn-(2) and anti-(3) conformation 8:1 for purified enzyme
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additional information
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no reduction of aldehydes or alcohols
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2 benzyl 2-methyl-3-oxobutyrate + 2 NADPH + 2 H+
benzyl (2R,3S)-2-methyl-3-hydroxybutanoate + benzyl (2S,3S)-2-methyl-3-hydroxybutanoate + 2 NADP+
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4.2
benzyl (2R,3S)-2-methyl-3-hydroxy-butanoate
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0.42
Benzyl 2-methyl-3-oxobutyrate
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4.2
benzyl(2S,3S)-2-methyl-3-hydroxybutanoate
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5 - 8
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less than 10% activity at pH 4 and 9
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brenda
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6 - 8
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purified enzyme at pH 6-8, rapid loss of activity below pH 6 and above pH 9
286280
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40
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rapid loss of activity above 40°C
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Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.
Purification and properties of an asymmetric reduction enzyme of 2-methyl-3-oxobutyrate in baker's yeast
Agric. Biol. Chem.
49
2563-2570
1985
Saccharomyces cerevisiae
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brenda
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